2i8e

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{{Seed}}
 
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[[Image:2i8e.png|left|200px]]
 
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==Structure of SSO1404, a predicted DNA repair-associated protein from Sulfolobus solfataricus P2==
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The line below this paragraph, containing "STRUCTURE_2i8e", creates the "Structure Box" on the page.
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<StructureSection load='2i8e' size='340' side='right'caption='[[2i8e]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2i8e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2I8E FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2i8e| PDB=2i8e | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2i8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i8e OCA], [https://pdbe.org/2i8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2i8e RCSB], [https://www.ebi.ac.uk/pdbsum/2i8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2i8e ProSAT], [https://www.topsan.org/Proteins/MCSG/2i8e TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAS2A_SACS2 CAS2A_SACS2] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Involved in the integration of spacer DNA into the CRISPR cassette (By similarity). Functions as a ssRNA-specific endoribonuclease, producing a 5'-phosphomonoester and a 3'-hydroxy. Does not process pre-crRNA in the manner expected if it were the CRISPR-processing endoribonuclease. Prefers U-rich substrates and often cuts between adjacent U residues in regions predicted to be single-stranded. RNAs as short as 10 residues can serve as substrate.<ref>PMID:18482976</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/2i8e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2i8e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Clustered regularly interspaced short palindromic repeats (CRISPRs) together with the associated CAS proteins protect microbial cells from invasion by foreign genetic elements using presently unknown molecular mechanisms. All CRISPR systems contain proteins of the CAS2 family, suggesting that these uncharacterized proteins play a central role in this process. Here we show that the CAS2 proteins represent a novel family of endoribonucleases. Six purified CAS2 proteins from diverse organisms cleaved single-stranded RNAs preferentially within U-rich regions. A representative CAS2 enzyme, SSO1404 from Sulfolobus solfataricus, cleaved the phosphodiester linkage on the 3'-side and generated 5'-phosphate- and 3'-hydroxyl-terminated oligonucleotides. The crystal structure of SSO1404 was solved at 1.6A resolution revealing the first ribonuclease with a ferredoxin-like fold. Mutagenesis of SSO1404 identified six residues (Tyr-9, Asp-10, Arg-17, Arg-19, Arg-31, and Phe-37) that are important for enzymatic activity and suggested that Asp-10 might be the principal catalytic residue. Thus, CAS2 proteins are sequence-specific endoribonucleases, and we propose that their role in the CRISPR-mediated anti-phage defense might involve degradation of phage or cellular mRNAs.
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===Structure of SSO1404, a predicted DNA repair-associated protein from Sulfolobus solfataricus P2===
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A novel family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats.,Beloglazova N, Brown G, Zimmerman MD, Proudfoot M, Makarova KS, Kudritska M, Kochinyan S, Wang S, Chruszcz M, Minor W, Koonin EV, Edwards AM, Savchenko A, Yakunin AF J Biol Chem. 2008 Jul 18;283(29):20361-71. Epub 2008 May 15. PMID:18482976<ref>PMID:18482976</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18482976}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2i8e" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18482976 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18482976}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2I8E is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus_p2 Sulfolobus solfataricus p2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I8E OCA].
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: Chruszcz M]]
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==Reference==
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[[Category: Edwards A]]
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<ref group="xtra">PMID:18482976</ref><references group="xtra"/>
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[[Category: Joachimiak A]]
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[[Category: Sulfolobus solfataricus p2]]
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[[Category: Kudritska M]]
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[[Category: Chruszcz, M.]]
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[[Category: Minor W]]
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[[Category: Edwards, A.]]
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[[Category: Savchenko A]]
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[[Category: Kudritska, M.]]
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[[Category: Wang S]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Zimmerman MD]]
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[[Category: Minor, W.]]
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[[Category: Savchenko, A.]]
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[[Category: Wang, S.]]
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[[Category: Zimmerman, M D.]]
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[[Category: Dna repair]]
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[[Category: Mcsg]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:05:54 2009''
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Current revision

Structure of SSO1404, a predicted DNA repair-associated protein from Sulfolobus solfataricus P2

PDB ID 2i8e

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