1cjb

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{{Seed}}
 
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[[Image:1cjb.png|left|200px]]
 
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==MALARIAL PURINE PHOSPHORIBOSYLTRANSFERASE==
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The line below this paragraph, containing "STRUCTURE_1cjb", creates the "Structure Box" on the page.
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<StructureSection load='1cjb' size='340' side='right'caption='[[1cjb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1cjb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. The July 2012 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Hypoxanthine-guanine phosphoribosyltransferase (HGPRT)'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2012_7 10.2210/rcsb_pdb/mom_2012_7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CJB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IRP:(1S)-1(9-DEAZAHYPOXANTHIN-9YL)1,4-DIDEOXY-1,4-IMINO-D-RIBITOL-5-PHOSPHATE'>IRP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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{{STRUCTURE_1cjb| PDB=1cjb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cjb OCA], [https://pdbe.org/1cjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cjb RCSB], [https://www.ebi.ac.uk/pdbsum/1cjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cjb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HGXR_PLAFG HGXR_PLAFG] Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Works with guanine, hypoxanthine and xanthine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cj/1cjb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cjb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Malaria is a leading cause of worldwide mortality from infectious disease. Plasmodium falciparum proliferation in human erythrocytes requires purine salvage by hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase). The enzyme is a target for the development of novel antimalarials. Design and synthesis of transition-state analogue inhibitors permitted cocrystallization with the malarial enzyme and refinement of the complex to 2.0 A resolution. Catalytic site contacts in the malarial enzyme are similar to those of human hypoxanthine-guanine phosphoribosyltransferase (HGPRTase) despite distinct substrate specificity. The crystal structure of malarial HGXPRTase with bound inhibitor, pyrophosphate, and two Mg(2+) ions reveals features unique to the transition-state analogue complex. Substrate-assisted catalysis occurs by ribooxocarbenium stabilization from the O5' lone pair and a pyrophosphate oxygen. A dissociative reaction coordinate path is implicated in which the primary reaction coordinate motion is the ribosyl C1' in motion between relatively immobile purine base and (Mg)(2)-pyrophosphate. Several short hydrogen bonds form in the complex of the enzyme and inhibitor. The proton NMR spectrum of the transition-state analogue complex of malarial HGXPRTase contains two downfield signals at 14.3 and 15.3 ppm. Despite the structural similarity to the human enzyme, the NMR spectra of the complexes reveal differences in hydrogen bonding between the transition-state analogue complexes of the human and malarial HG(X)PRTases. The X-ray crystal structures and NMR spectra reveal chemical and structural features that suggest a strategy for the design of malaria-specific transition-state inhibitors.
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===MALARIAL PURINE PHOSPHORIBOSYLTRANSFERASE===
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The 2.0 A structure of malarial purine phosphoribosyltransferase in complex with a transition-state analogue inhibitor.,Shi W, Li CM, Tyler PC, Furneaux RH, Cahill SM, Girvin ME, Grubmeyer C, Schramm VL, Almo SC Biochemistry. 1999 Aug 3;38(31):9872-80. PMID:10433693<ref>PMID:10433693</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1cjb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10433693}}, adds the Publication Abstract to the page
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*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10433693 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10433693}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1CJB is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CJB OCA].
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==Reference==
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<ref group="xtra">PMID:10433693</ref><references group="xtra"/>
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[[Category: Hypoxanthine phosphoribosyltransferase]]
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[[Category: Plasmodium falciparum]]
[[Category: Plasmodium falciparum]]
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[[Category: Almo, S C.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Cahill, S M.]]
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[[Category: Almo SC]]
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[[Category: Furneaux, R H.]]
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[[Category: Cahill SM]]
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[[Category: Girvin, M E.]]
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[[Category: Furneaux RH]]
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[[Category: Grubmeyer, C.]]
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[[Category: Girvin ME]]
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[[Category: Li, C M.]]
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[[Category: Grubmeyer C]]
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[[Category: Schramm, V L.]]
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[[Category: Li CM]]
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[[Category: Shi, W.]]
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[[Category: Schramm VL]]
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[[Category: Tyler, P C.]]
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[[Category: Shi W]]
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[[Category: Malaria]]
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[[Category: Tyler PC]]
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[[Category: Phosphoribosyltransferase]]
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[[Category: Purine salvage]]
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[[Category: Transition state inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:22:19 2009''
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Current revision

MALARIAL PURINE PHOSPHORIBOSYLTRANSFERASE

PDB ID 1cjb

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