2g2q

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{{Seed}}
 
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[[Image:2g2q.png|left|200px]]
 
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==The crystal structure of G4, the poxviral disulfide oxidoreductase essential for cytoplasmic disulfide bond formation==
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The line below this paragraph, containing "STRUCTURE_2g2q", creates the "Structure Box" on the page.
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<StructureSection load='2g2q' size='340' side='right'caption='[[2g2q]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2g2q]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G2Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2g2q| PDB=2g2q | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g2q OCA], [https://pdbe.org/2g2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g2q RCSB], [https://www.ebi.ac.uk/pdbsum/2g2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g2q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLRX2_VACCW GLRX2_VACCW] Glutaredoxin necessary for virion morphogenesis and virus replication. Functions as a thiol-disulfide transfer protein between membrane-associated A2.5 and substrates L1 or F9. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves oxidation of E10, A2.5 and G4. Exhibit thioltransferase and dehydroascorbate reductase activities in vitro.<ref>PMID:10982364</ref> <ref>PMID:11752136</ref> <ref>PMID:11983854</ref> <ref>PMID:8955061</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g2/2g2q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g2q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The possibility of the release of smallpox virus into a predominantly nonimmunized population highlights the importance of understanding poxvirus biology. Poxviruses encode a conserved pathway that is required to oxidize disulfide bonds in nascent viral proteins that fold in the reducing environment of the eukaryotic host cytoplasm. We present the structure of the last enzyme of the vaccinia virus pathway, G4, which is almost identical in smallpox virus. G4 catalyzes the formation of disulfide bonds in proteins that are critical for virus maturation and host cell infection. G4 contains a thioredoxin fold and a Cys-X-X-Cys active site. In solution, G4 monomers and dimers are observed. In the crystal, G4 is found as a dimer that buries 4,500 A(2) in the interface and occludes the active site, which could protect the reactive disulfide from reduction in the cytoplasm. The structure serves as a model for drug design targeting viral disulfide bond formation.
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===The crystal structure of G4, the poxviral disulfide oxidoreductase essential for cytoplasmic disulfide bond formation===
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The structure of G4, the poxvirus disulfide oxidoreductase essential for virus maturation and infectivity.,Su HP, Lin DY, Garboczi DN J Virol. 2006 Aug;80(15):7706-13. PMID:16840349<ref>PMID:16840349</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 2g2q" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16840349 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16840349}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2G2Q is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G2Q OCA].
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==Reference==
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<ref group="xtra">PMID:16840349</ref><references group="xtra"/>
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[[Category: Vaccinia virus]]
[[Category: Vaccinia virus]]
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[[Category: Garboczi, D N.]]
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[[Category: Garboczi DN]]
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[[Category: Lin, D Y.]]
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[[Category: Lin DY]]
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[[Category: Su, H P.]]
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[[Category: Su HP]]
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[[Category: G4]]
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[[Category: Orthopox]]
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[[Category: Oxidoreductase]]
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[[Category: Poxvirus]]
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[[Category: Thioredoxin-fold]]
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[[Category: Vaccinia virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:44:47 2009''
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Current revision

The crystal structure of G4, the poxviral disulfide oxidoreductase essential for cytoplasmic disulfide bond formation

PDB ID 2g2q

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