2jjx

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{{Seed}}
 
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[[Image:2jjx.png|left|200px]]
 
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==THE CRYSTAL STRUCTURE OF UMP KINASE FROM BACILLUS ANTHRACIS (BA1797)==
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The line below this paragraph, containing "STRUCTURE_2jjx", creates the "Structure Box" on the page.
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<StructureSection load='2jjx' size='340' side='right'caption='[[2jjx]], [[Resolution|resolution]] 2.82&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jjx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._Ames Bacillus anthracis str. Ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JJX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.82&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_2jjx| PDB=2jjx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jjx OCA], [https://pdbe.org/2jjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jjx RCSB], [https://www.ebi.ac.uk/pdbsum/2jjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jjx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A6L7HKK4_BACAN A0A6L7HKK4_BACAN] Catalyzes the reversible phosphorylation of UMP to UDP.[HAMAP-Rule:MF_01220]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/2jjx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jjx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Uridine monophosphate (UMP) kinase is a conserved enzyme that catalyzes the ATP-driven conversion of uridylate monophosphate into uridylate diphosphate, an essential metabolic step. In prokaryotes, the enzyme exists as a homohexamer that is regulated by various metabolites. Whereas the enzymatic mechanism of UMP kinase (UK) is well-characterized, the molecular basis of its regulation remains poorly understood. Here we report the crystal structure of UK from Bacillus anthracis (BA1797) in complex with ATP at 2.82 A resolution. It reveals that the cofactor, in addition to binding in the active sites, also interacts with separate binding pockets located near the center of the hexameric structure. The existence of such an allosteric binding site had been predicted by biochemical studies, but it was not identified in previous crystal structures of prokaryotic UKs. We show that this putative allosteric pocket is conserved across different bacterial species, suggesting that it is a feature common to bacterial UKs, and we present a structural model for the allosteric regulation of this enzyme.
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===THE CRYSTAL STRUCTURE OF UMP KINASE FROM BACILLUS ANTHRACIS (BA1797)===
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The crystal structure of UMP kinase from Bacillus anthracis (BA1797) reveals an allosteric nucleotide-binding site.,Meier C, Carter LG, Sainsbury S, Mancini EJ, Owens RJ, Stuart DI, Esnouf RM J Mol Biol. 2008 Sep 19;381(5):1098-105. Epub 2008 Jul 3. PMID:18625239<ref>PMID:18625239</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2jjx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18625239}}, adds the Publication Abstract to the page
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*[[Uridylate kinase|Uridylate kinase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18625239 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18625239}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus anthracis str. Ames]]
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2JJX is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JJX OCA].
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[[Category: Large Structures]]
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[[Category: Carter LG]]
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==Reference==
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[[Category: Esnouf RM]]
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<ref group="xtra">PMID:18625239</ref><references group="xtra"/>
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[[Category: Mancini EJ]]
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[[Category: Bacillus anthracis]]
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[[Category: Meier C]]
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[[Category: UMP kinase]]
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[[Category: Owens RJ]]
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[[Category: Carter, L G.]]
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[[Category: Stuart DI]]
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[[Category: Esnouf, R M.]]
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[[Category: Mancini, E J.]]
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[[Category: Meier, C.]]
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[[Category: Oppf, Oxford Protein Production Facility.]]
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[[Category: Owens, R J.]]
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[[Category: Spine, Structural Proteomics in Europe.]]
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[[Category: Stuart, D I.]]
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[[Category: Atp-binding]]
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[[Category: Cytoplasm]]
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[[Category: Kinase]]
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[[Category: Nucleotide-binding]]
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[[Category: Oppf]]
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[[Category: Pyrh]]
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[[Category: Pyrimidine biosynthesis]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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[[Category: Uridylate kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:56:03 2009''
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Current revision

THE CRYSTAL STRUCTURE OF UMP KINASE FROM BACILLUS ANTHRACIS (BA1797)

PDB ID 2jjx

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