2jkx

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{{Seed}}
 
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[[Image:2jkx.png|left|200px]]
 
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==Galactose oxidase. MatGO. Copper free, expressed in Pichia Pastoris.==
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The line below this paragraph, containing "STRUCTURE_2jkx", creates the "Structure Box" on the page.
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<StructureSection load='2jkx' size='340' side='right'caption='[[2jkx]], [[Resolution|resolution]] 1.84&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2jkx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_graminearum Fusarium graminearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JKX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.84&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_2jkx| PDB=2jkx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jkx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jkx OCA], [https://pdbe.org/2jkx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jkx RCSB], [https://www.ebi.ac.uk/pdbsum/2jkx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jkx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAOA_GIBZA GAOA_GIBZA] Catalyzes the sterospecific oxidation of primary alcohols to the corresponding aldehydes. The biologically relevant substrate of the enzyme is not known as the enzyme exhibits broad substrate specificity from small alcohols through sugars to oligo- and polysaccharides.<ref>PMID:13641238</ref> <ref>PMID:4441089</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/2jkx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jkx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Galactose oxidase (GO; EC 1.1.3.9) catalyses the oxidation of a wide range of primary alcohols including mono-, oligo- and polysaccharides. High-resolution structures have been determined for GO, but no structural information is available for the enzyme with bound substrate or inhibitor. Previously, computer-aided docking experiments have been used to develop a plausible model for interactions between GO and the D-galactose substrate. Residues implicated in such interactions include Arg330, Gln406, Phe464, Phe194 and Trp290. In the present study we describe an improved expression system for recombinant GO in the methylotrophic yeast Pichia pastoris. We use this system to express variant proteins mutated at Arg330 and Phe464 to explore the substrate binding model. We also demonstrate that the Arg330 variants display greater fructose oxidase activity than does wild-type GO.
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===GALACTOSE OXIDASE. MATGO. COPPER FREE, EXPRESSED IN PICHIA PASTORIS.===
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Enhanced fructose oxidase activity in a galactose oxidase variant.,Deacon SE, Mahmoud K, Spooner RK, Firbank SJ, Knowles PF, Phillips SE, McPherson MJ Chembiochem. 2004 Jul 5;5(7):972-9. PMID:15239055<ref>PMID:15239055</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2jkx" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15239055}}, adds the Publication Abstract to the page
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*[[Galactose oxidase|Galactose oxidase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15239055 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15239055}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Fusarium graminearum]]
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2JKX is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Gibberella_zeae Gibberella zeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JKX OCA].
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[[Category: Large Structures]]
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[[Category: Deacon SE]]
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==Reference==
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[[Category: Firbank SJ]]
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<ref group="xtra">PMID:15239055</ref><references group="xtra"/>
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[[Category: Knowles PF]]
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[[Category: Galactose oxidase]]
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[[Category: Mahmoud K]]
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[[Category: Gibberella zeae]]
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[[Category: McPherson MJ]]
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[[Category: Deacon, S E.]]
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[[Category: Phillips SEV]]
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[[Category: Firbank, S J.]]
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[[Category: Spooner RK]]
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[[Category: Knowles, P F.]]
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[[Category: Mahmoud, K.]]
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[[Category: McPherson, M J.]]
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[[Category: Phillips, S E.V.]]
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[[Category: Spooner, R K.]]
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[[Category: Copper]]
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[[Category: Copper enzyme]]
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[[Category: Enzyme catalysis]]
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[[Category: Kelch repeat]]
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[[Category: Metal-binding]]
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[[Category: Oxidase]]
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[[Category: Oxidoreductase]]
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[[Category: Protein engineering]]
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[[Category: Secreted]]
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[[Category: Thioether bond]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 15:58:58 2009''
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Current revision

Galactose oxidase. MatGO. Copper free, expressed in Pichia Pastoris.

PDB ID 2jkx

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