1iup

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{{Seed}}
 
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[[Image:1iup.png|left|200px]]
 
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==meta-Cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) S103A mutant complexed with isobutyrates==
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The line below this paragraph, containing "STRUCTURE_1iup", creates the "Structure Box" on the page.
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<StructureSection load='1iup' size='340' side='right'caption='[[1iup]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1iup]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IUP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IUP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALQ:2-METHYL-PROPIONIC+ACID'>ALQ</scene></td></tr>
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{{STRUCTURE_1iup| PDB=1iup | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iup OCA], [https://pdbe.org/1iup PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iup RCSB], [https://www.ebi.ac.uk/pdbsum/1iup PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iup ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/P96965_PSEFL P96965_PSEFL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/1iup_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iup ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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2-Hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase (CumD) from Pseudomonas fluorescens IP01 hydrolyzes a meta-cleavage product generated in the cumene (isopropylbenzene) degradation pathway. The crystal structures of the inactive S103A mutant of the CumD enzyme complexed with isobutyrate and acetate ions were determined at 1.6 and 2.0 A resolution, respectively. The isobutyrate and acetate ions were located at the same position in the active site, and occupied the site for a part of the hydrolysis product with CumD, which has the key determinant group for the substrate specificity of related hydrolases. One of the oxygen atoms of the carboxyl group of the isobutyrate ion was hydrogen bonded with a water molecule and His252. Another oxygen atom of the carboxyl group was situated in an oxyanion hole formed by the two main-chain N atoms. The isopropyl group of the isobutyric acid was recognized by the side-chains of the hydrophobic residues. The substrate-binding pocket of CumD was long, and the inhibition constants of various organic acids corresponded well to it. In comparison with the structure of BphD from Rhodococcus sp. RHA1, the structural basis for the substrate specificity of related hydrolases, is revealed.
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===meta-Cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) S103A mutant complexed with isobutyrates===
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Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products.,Fushinobu S, Saku T, Hidaka M, Jun SY, Nojiri H, Yamane H, Shoun H, Omori T, Wakagi T Protein Sci. 2002 Sep;11(9):2184-95. PMID:12192074<ref>PMID:12192074</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12192074}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1iup" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12192074 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12192074}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1IUP is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IUP OCA].
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==Reference==
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<ref group="xtra">PMID:12192074</ref><references group="xtra"/>
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[[Category: 2-hydroxymuconate-semialdehyde hydrolase]]
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[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
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[[Category: Fushinobu, S.]]
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[[Category: Fushinobu S]]
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[[Category: Hidaka, M.]]
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[[Category: Hidaka M]]
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[[Category: Jun, S Y.]]
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[[Category: Jun S-Y]]
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[[Category: Nojiri, H.]]
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[[Category: Nojiri H]]
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[[Category: Omori, T.]]
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[[Category: Omori T]]
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[[Category: Saku, T.]]
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[[Category: Saku T]]
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[[Category: Shoun, H.]]
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[[Category: Shoun H]]
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[[Category: Wakagi, T.]]
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[[Category: Wakagi T]]
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[[Category: Yamane, H.]]
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[[Category: Yamane H]]
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[[Category: Alpha/beta-hydrolase]]
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[[Category: Aromatic compound]]
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[[Category: Beta-ketolase]]
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[[Category: Cumene]]
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[[Category: Cumene degradation]]
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[[Category: Isopropylbenzene]]
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[[Category: Meta-cleavage compound hydrolase]]
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[[Category: Pcb]]
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[[Category: Polychlorinated biphenyl degradation]]
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[[Category: Pseudomonas fluorescens ip01]]
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[[Category: Substrate specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:08:56 2009''
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Current revision

meta-Cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) S103A mutant complexed with isobutyrates

PDB ID 1iup

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