1jty

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{{Seed}}
 
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[[Image:1jty.png|left|200px]]
 
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==Crystal structure of the multidrug binding transcriptional regulator QacR bound to ethidium==
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The line below this paragraph, containing "STRUCTURE_1jty", creates the "Structure Box" on the page.
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<StructureSection load='1jty' size='340' side='right'caption='[[1jty]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1jty]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JTY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.97&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1jty| PDB=1jty | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jty OCA], [https://pdbe.org/1jty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jty RCSB], [https://www.ebi.ac.uk/pdbsum/1jty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jty ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/QACR_STAAU QACR_STAAU] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jty_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jty ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Staphylococcus aureus multidrug binding protein QacR represses transcription of the qacA multidrug transporter gene and is induced by structurally diverse cationic lipophilic drugs. Here, we report the crystal structures of six QacR-drug complexes. Compared to the DNA bound structure, drug binding elicits a coil-to-helix transition that causes induction and creates an expansive multidrug-binding pocket, containing four glutamates and multiple aromatic and polar residues. These structures indicate the presence of separate but linked drug-binding sites within a single protein. This multisite drug-binding mechanism is consonant with studies on multidrug resistance transporters.
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===Crystal structure of the multidrug binding transcriptional regulator QacR bound to ethidium===
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Structural mechanisms of QacR induction and multidrug recognition.,Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG Science. 2001 Dec 7;294(5549):2158-63. PMID:11739955<ref>PMID:11739955</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11739955}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1jty" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11739955 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11739955}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1JTY is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTY OCA].
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==Reference==
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<ref group="xtra">PMID:11739955</ref><references group="xtra"/>
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Brennan, R G.]]
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[[Category: Brennan RG]]
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[[Category: Brown, M H.]]
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[[Category: Brown MH]]
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[[Category: Grkovic, S.]]
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[[Category: Grkovic S]]
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[[Category: Miller, M C.]]
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[[Category: Miller MC]]
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[[Category: Schumacher, M A.]]
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[[Category: Schumacher MA]]
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[[Category: Skurray, R A.]]
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[[Category: Skurray RA]]
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[[Category: Cationic lipophilic drug]]
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[[Category: Multidrug binding]]
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[[Category: Multidrug recognition mechanism]]
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[[Category: Qaca]]
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[[Category: Qacr]]
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[[Category: Repressor]]
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[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:11:14 2009''
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Current revision

Crystal structure of the multidrug binding transcriptional regulator QacR bound to ethidium

PDB ID 1jty

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