1e96

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{{Seed}}
 
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[[Image:1e96.png|left|200px]]
 
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==Structure of the Rac/p67phox complex==
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The line below this paragraph, containing "STRUCTURE_1e96", creates the "Structure Box" on the page.
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<StructureSection load='1e96' size='340' side='right'caption='[[1e96]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1e96]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E96 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E96 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1e96| PDB=1e96 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e96 OCA], [https://pdbe.org/1e96 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e96 RCSB], [https://www.ebi.ac.uk/pdbsum/1e96 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e96 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RAC1_HUMAN RAC1_HUMAN] Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref> Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.<ref>PMID:1643658</ref> <ref>PMID:9121475</ref> <ref>PMID:19934221</ref> <ref>PMID:19403692</ref> <ref>PMID:20696765</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e9/1e96_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e96 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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p67phox is an essential part of the NADPH oxidase, a multiprotein enzyme complex that produces superoxide ions in response to microbial infection. Binding of the small GTPase Rac to p67phox is a key step in the assembly of the active enzyme complex. The structure of Rac.GTP bound to the N-terminal TPR (tetratricopeptide repeat) domain of p67phox reveals a novel mode of Rho family/effector interaction and explains the basis of GTPase specificity. Complex formation is largely mediated by an insertion between two TPR motifs, suggesting an unsuspected versatility of TPR domains in target recognition and in their more general role as scaffolds for the assembly of multiprotein complexes.
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===STRUCTURE OF THE RAC/P67PHOX COMPLEX===
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Structure of the TPR domain of p67phox in complex with Rac.GTP.,Lapouge K, Smith SJ, Walker PA, Gamblin SJ, Smerdon SJ, Rittinger K Mol Cell. 2000 Oct;6(4):899-907. PMID:11090627<ref>PMID:11090627</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1e96" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11090627}}, adds the Publication Abstract to the page
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*[[NADPH oxidase 3D structures|NADPH oxidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11090627 is the PubMed ID number.
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*[[Rac 3D structures|Rac 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_11090627}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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1E96 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E96 OCA].
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==Reference==
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<ref group="xtra">PMID:11090627</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Gamblin, S J.]]
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[[Category: Large Structures]]
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[[Category: Lapouge, K.]]
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[[Category: Gamblin SJ]]
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[[Category: Rittinger, K.]]
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[[Category: Lapouge K]]
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[[Category: Smerdon, S J.]]
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[[Category: Rittinger K]]
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[[Category: Smith, S J.M.]]
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[[Category: Smerdon SJ]]
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[[Category: Walker, P A.]]
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[[Category: Smith SJM]]
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[[Category: Gtpase]]
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[[Category: Walker PA]]
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[[Category: Nadph oxidase]]
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[[Category: Protein-protein complex]]
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[[Category: Signalling complex]]
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[[Category: Tpr motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:42:43 2009''
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Current revision

Structure of the Rac/p67phox complex

PDB ID 1e96

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