1eii

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{{Seed}}
 
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[[Image:1eii.png|left|200px]]
 
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==NMR STRUCTURE OF HOLO CELLULAR RETINOL-BINDING PROTEIN II==
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The line below this paragraph, containing "STRUCTURE_1eii", creates the "Structure Box" on the page.
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<StructureSection load='1eii' size='340' side='right'caption='[[1eii]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1eii]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EII FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RTL:RETINOL'>RTL</scene></td></tr>
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{{STRUCTURE_1eii| PDB=1eii | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1eii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1eii OCA], [https://pdbe.org/1eii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1eii RCSB], [https://www.ebi.ac.uk/pdbsum/1eii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1eii ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RET2_RAT RET2_RAT] Intracellular transport of retinol.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1eii_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1eii ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure and backbone dynamics of rat holo cellular retinol-binding protein II (holo-CRBP II) in solution has been determined by multidimensional NMR. The final structure ensemble was based on 3980 distance and 30 dihedral angle restraints, and was calculated using metric matrix distance geometry with pairwise Gaussian metrization followed by simulated annealing. The average RMS deviation of the backbone atoms for the final 25 structures relative to their mean coordinates is 0.85(+/-0.09) A. Comparison of the solution structure of holo-CRBP II with apo-CRBP II indicates that the protein undergoes conformational changes not previously observed in crystalline CRBP II, affecting residues 28-35 of the helix-turn-helix, residues 37-38 of the subsequent linker, as well as the beta-hairpin C-D, E-F and G-H loops. The bound retinol is completely buried inside the binding cavity and oriented as in the crystal structure. The order parameters derived from the (15)N T(1), T(2) and steady-state NOE parameters show that the backbone dynamics of holo-CRBP II is restricted throughout the polypeptide. The T(2) derived apparent backbone exchange rate and amide (1)H exchange rate both indicate that the microsecond to second timescale conformational exchange occurring in the portal region of the apo form has been suppressed in the holo form.
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===NMR STRUCTURE OF HOLO CELLULAR RETINOL-BINDING PROTEIN II===
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Binding of retinol induces changes in rat cellular retinol-binding protein II conformation and backbone dynamics.,Lu J, Lin CL, Tang C, Ponder JW, Kao JL, Cistola DP, Li E J Mol Biol. 2000 Jul 14;300(3):619-32. PMID:10884357<ref>PMID:10884357</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1eii" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10884357}}, adds the Publication Abstract to the page
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*[[Retinol-binding protein 3D structures|Retinol-binding protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10884357 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10884357}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1EII is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EII OCA].
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==Reference==
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<ref group="xtra">PMID:10884357</ref><references group="xtra"/>
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Cistola, D P.]]
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[[Category: Cistola DP]]
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[[Category: Kao, J L.]]
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[[Category: Kao JL]]
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[[Category: Li, E.]]
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[[Category: Li E]]
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[[Category: Lin, C L.]]
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[[Category: Lin CL]]
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[[Category: Lu, J.]]
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[[Category: Lu J]]
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[[Category: Ponder, J W.]]
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[[Category: Ponder JW]]
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[[Category: Tang, C.]]
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[[Category: Tang C]]
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[[Category: Beta barrel]]
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[[Category: Helix-turn-helix]]
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[[Category: Protein-ligand complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:52:45 2009''
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Current revision

NMR STRUCTURE OF HOLO CELLULAR RETINOL-BINDING PROTEIN II

PDB ID 1eii

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