2gz6

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{{Seed}}
 
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[[Image:2gz6.png|left|200px]]
 
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==Crystal Structure Of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase At 2.0 A==
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The line below this paragraph, containing "STRUCTURE_2gz6", creates the "Structure Box" on the page.
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<StructureSection load='2gz6' size='340' side='right'caption='[[2gz6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gz6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anabaena_sp._CH1 Anabaena sp. CH1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GZ6 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gz6 OCA], [https://pdbe.org/2gz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gz6 RCSB], [https://www.ebi.ac.uk/pdbsum/2gz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gz6 ProSAT]</span></td></tr>
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{{STRUCTURE_2gz6| PDB=2gz6 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A4UA16_9NOST A4UA16_9NOST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gz/2gz6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gz6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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N-acetyl-D-glucosamine 2-epimerase (GlcNAc 2-epimerase) catalyzes the reversible epimerization between N-acetyl-D-glucosamine (GlcNAc) and N-acetyl-D-mannosamine (ManNAc). We report here the 2.0 A resolution crystal structure of the GlcNAc 2-epimerase from Anabaena sp. CH1. The structure demonstrates an (alpha/alpha)(6) barrel fold, which shows structural homology with porcine GlcNAc 2-epimerase as well as a number of glycoside hydrolase enzymes and other sugar-metabolizing enzymes. One side of the barrel structure consists of short loops involved in dimer interactions. The other side of the barrel structure is comprised of long loops containing six short beta-sheets, which enclose a putative central active-site pocket. Site-directed mutagenesis of conserved residues near the N-terminal region of the inner alpha helices shows that R57, H239, E308, and H372 are strictly required for activity. E242 and R375 are also essential in catalysis. Based on the structure and kinetic analysis, H239 and H372 may serve as the key active site acid/base catalysts. These results suggest that the (alpha/alpha)(6) barrel represents a steady fold for presenting active-site residues in a cleft at the N-terminal ends of the inner alpha helices, thus forming a fine-tuned catalytic site in GlcNAc 2-epimerase.
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===Crystal Structure Of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase At 2.0 A===
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The central cavity from the (alpha/alpha)6 barrel structure of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase contains two key histidine residues for reversible conversion.,Lee YC, Wu HM, Chang YN, Wang WC, Hsu WH J Mol Biol. 2007 Mar 30;367(3):895-908. Epub 2006 Nov 6. PMID:17292397<ref>PMID:17292397</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17292397}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2gz6" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17292397 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17292397}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Anabaena sp. CH1]]
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2GZ6 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GZ6 OCA].
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[[Category: Large Structures]]
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[[Category: Chang YN]]
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==Reference==
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[[Category: Wang WC]]
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<ref group="xtra">PMID:17292397</ref><references group="xtra"/>
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[[Category: Wu HM]]
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[[Category: Anabaena sp.]]
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[[Category: Mannose-6-phosphate isomerase]]
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[[Category: Chang, Y N.]]
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[[Category: Wang, W C.]]
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[[Category: Wu, H M.]]
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[[Category: Anabaena sp. ch1]]
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[[Category: N-acetyl-d-glucosamine 2-epimerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:57:40 2009''
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Current revision

Crystal Structure Of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase At 2.0 A

PDB ID 2gz6

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