1azo

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{{Seed}}
 
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[[Image:1azo.png|left|200px]]
 
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==DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI==
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The line below this paragraph, containing "STRUCTURE_1azo", creates the "Structure Box" on the page.
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<StructureSection load='1azo' size='340' side='right'caption='[[1azo]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1azo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AZO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1azo| PDB=1azo | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1azo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1azo OCA], [https://pdbe.org/1azo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1azo RCSB], [https://www.ebi.ac.uk/pdbsum/1azo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1azo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTH_ECOLI MUTH_ECOLI] Sequence-specific endonuclease that cleaves unmethylated GATC sequences. It is involved in DNA mismatch repair.[HAMAP-Rule:MF_00759]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/1azo_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1azo ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MutS, MutL and MutH are the three essential proteins for initiation of methyl-directed DNA mismatch repair to correct mistakes made during DNA replication in Escherichia coli. MutH cleaves a newly synthesized and unmethylated daughter strand 5' to the sequence d(GATC) in a hemi-methylated duplex. Activation of MutH requires the recognition of a DNA mismatch by MutS and MutL. We have crystallized MutH in two space groups and solved the structures at 1.7 and 2.3 A resolution, respectively. The active site of MutH is located at an interface between two subdomains that pivot relative to one another, as revealed by comparison of the crystal structures, and this presumably regulates the nuclease activity. The relative motion of the two subdomains in MutH correlates with the position of a protruding C-terminal helix. This helix appears to act as a molecular lever through which MutS and MutL may communicate the detection of a DNA mismatch and activate MutH. With sequence homology to Sau3AI and structural similarity to PvuII endonuclease, MutH is clearly related to these enzymes by divergent evolution, and this suggests that type II restriction endonucleases evolved from a common ancestor.
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===DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI===
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Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases.,Ban C, Yang W EMBO J. 1998 Mar 2;17(5):1526-34. PMID:9482749<ref>PMID:9482749</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1azo" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9482749}}, adds the Publication Abstract to the page
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9482749 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9482749}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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1AZO is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AZO OCA].
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[[Category: Large Structures]]
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[[Category: Yang W]]
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==Reference==
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<ref group="xtra">PMID:9482749</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
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[[Category: Yang, W.]]
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[[Category: Dna repair]]
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[[Category: Endonuclease]]
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[[Category: Hydrolase]]
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[[Category: Muth]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 16:57:44 2009''
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Current revision

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

PDB ID 1azo

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