2ext

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(New page: 200px<br /><applet load="2ext" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ext, resolution 1.80&Aring;" /> '''TRAP4 (engineered TR...)
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[[Image:2ext.gif|left|200px]]<br /><applet load="2ext" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ext, resolution 1.80&Aring;" />
 
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'''TRAP4 (engineered TRAP)'''<br />
 
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==Overview==
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==TRAP4 (engineered TRAP)==
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The protein TRAP (trp RNA binding attenuation protein) forms a highly, thermostable ring-shaped 11-mer. By linking in tandem two, three, or four, DNA sequences encoding TRAP monomers, we have engineered new rings that, consist of 12 TRAP subunits and bind 12 ligand molecules. The hydrogen, bonding pattern and buried surface area within and between subunits are, essentially identical between the 11-mer and 12-mer crystal structures., Why do the artificial proteins choose to make single 12-mer rings? The, 12-mer rings are highly sterically strained by their peptide linkers and, far from thermostable. That proteins choose to adopt a strained, conformation of few subunits rather than an unstrained one with 11, subunits demonstrates the importance of entropic factors in controlling, protein-protein interactions in general.
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<StructureSection load='2ext' size='340' side='right'caption='[[2ext]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ext]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EXT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ext FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ext OCA], [https://pdbe.org/2ext PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ext RCSB], [https://www.ebi.ac.uk/pdbsum/2ext PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ext ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MTRB_GEOSE MTRB_GEOSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/2ext_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ext ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2EXT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with TRP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2EXT OCA].
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*[[Tryptophan RNA-binding attenuation protein|Tryptophan RNA-binding attenuation protein]]
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__TOC__
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==Reference==
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</StructureSection>
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Rounding up: Engineering 12-membered rings from the cyclic 11-mer TRAP., Heddle JG, Yokoyama T, Yamashita I, Park SY, Tame JR, Structure. 2006 May;14(5):925-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16698553 16698553]
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[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Heddle, J.G.]]
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[[Category: Heddle JG]]
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[[Category: Park, S.Y.]]
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[[Category: Park SY]]
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[[Category: Tame, J.R.H.]]
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[[Category: Tame JRH]]
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[[Category: Yamashita, I.]]
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[[Category: Yamashita I]]
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[[Category: Yokoyama, T.]]
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[[Category: Yokoyama T]]
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[[Category: TRP]]
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[[Category: 12-mer]]
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[[Category: artificial]]
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[[Category: engineered]]
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[[Category: ring protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:12:36 2007''
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Current revision

TRAP4 (engineered TRAP)

PDB ID 2ext

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