1nx8

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{{Seed}}
 
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[[Image:1nx8.png|left|200px]]
 
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==Structure of carbapenem synthase (CarC) complexed with N-acetyl proline==
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The line below this paragraph, containing "STRUCTURE_1nx8", creates the "Structure Box" on the page.
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<StructureSection load='1nx8' size='340' side='right'caption='[[1nx8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nx8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NX8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AKG:2-OXOGLUTARIC+ACID'>AKG</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=N7P:1-ACETYL-L-PROLINE'>N7P</scene></td></tr>
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{{STRUCTURE_1nx8| PDB=1nx8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx8 OCA], [https://pdbe.org/1nx8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nx8 RCSB], [https://www.ebi.ac.uk/pdbsum/1nx8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nx8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CARC_PECCC CARC_PECCC] Catalyzes the Fe(2+) and alpha-ketoglutarate-dependent conversion of (3S,5S)-carbapenam to (5R)-carbapenem, an essential step in carbapenem antibiotic biosynthesis.<ref>PMID:9402024</ref> <ref>PMID:12848554</ref> <ref>PMID:15174175</ref> <ref>PMID:12611886</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nx/1nx8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nx8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The proposed biosynthetic pathway to the carbapenem antibiotics proceeds via epimerization/desaturation of a carbapenam in an unusual process catalyzed by an iron- and 2-oxoglutarate-dependent oxygenase, CarC. Crystal structures of CarC complexed with Fe(II) and 2-oxoglutarate reveal it to be hexameric (space group C2221), consistent with solution studies. CarC monomers contain a double-stranded beta-helix core that supports ligands binding a single Fe(II) to which 2-oxoglutarate complexes in a bi-dentate manner. A structure was obtained with l-N-acetylproline acting as a substrate analogue. Quantum mechanical/molecular mechanical modeling studies with stereoisomers of carbapenams and carbapenems were used to investigate substrate binding. The combined work will stimulate further mechanistic studies and aid in the engineering of carbapenem biosynthesis.
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===Structure of carbapenem synthase (CarC) complexed with N-acetyl proline===
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Crystal structure of carbapenem synthase (CarC).,Clifton IJ, Doan LX, Sleeman MC, Topf M, Suzuki H, Wilmouth RC, Schofield CJ J Biol Chem. 2003 Jun 6;278(23):20843-50. Epub 2003 Feb 28. PMID:12611886<ref>PMID:12611886</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12611886}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1nx8" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12611886 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12611886}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1NX8 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Pectobacterium_carotovorum Pectobacterium carotovorum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX8 OCA].
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==Reference==
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<ref group="xtra">PMID:12611886</ref><references group="xtra"/>
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[[Category: Pectobacterium carotovorum]]
[[Category: Pectobacterium carotovorum]]
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[[Category: Clifton, I J.]]
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[[Category: Clifton IJ]]
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[[Category: Doan, L X.]]
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[[Category: Doan LX]]
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[[Category: Schofield, C J.]]
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[[Category: Schofield CJ]]
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[[Category: Sleeman, M C.]]
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[[Category: Sleeman MC]]
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[[Category: Suzuki, H.]]
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[[Category: Suzuki H]]
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[[Category: Topf, M.]]
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[[Category: Topf M]]
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[[Category: Wilmouth, R C.]]
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[[Category: Wilmouth RC]]
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[[Category: Jelly roll]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:09:29 2009''
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Current revision

Structure of carbapenem synthase (CarC) complexed with N-acetyl proline

PDB ID 1nx8

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