1b0a

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{{Seed}}
 
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[[Image:1b0a.png|left|200px]]
 
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==5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.==
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The line below this paragraph, containing "STRUCTURE_1b0a", creates the "Structure Box" on the page.
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<StructureSection load='1b0a' size='340' side='right'caption='[[1b0a]], [[Resolution|resolution]] 2.56&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1b0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1B0A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.56&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1b0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1b0a OCA], [https://pdbe.org/1b0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1b0a RCSB], [https://www.ebi.ac.uk/pdbsum/1b0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1b0a ProSAT]</span></td></tr>
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{{STRUCTURE_1b0a| PDB=1b0a | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FOLD_ECOLI FOLD_ECOLI] Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.<ref>PMID:1748668</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b0/1b0a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1b0a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of a bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/cyclohydrolase from Escherichia coli has been determined at 2.5 A resolution in the absence of bound substrates and compared to the NADP-bound structure of the homologous enzyme domains from a trifunctional human synthetase enzyme. Superposition of these structures allows the identification of a highly conserved cluster of basic residues that are appropriately positioned to serve as a binding site for the poly-gamma-glutamyl tail of the tetrahydrofolate substrate. Modeling studies and molecular dynamic simulations of bound methylene-tetrahydrofolate and NADP shows that this binding site would allow interaction of the nicotinamide and pterin rings in the dehydrogenase active site. Comparison of these enzymes also indicates differences between their active sites that might allow the development of inhibitors specific to the bacterial target.
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===5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.===
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The crystal structure of a bacterial, bifunctional 5,10 methylene-tetrahydrofolate dehydrogenase/cyclohydrolase.,Shen BW, Dyer DH, Huang JY, D'Ari L, Rabinowitz J, Stoddard BL Protein Sci. 1999 Jun;8(6):1342-9. PMID:10386884<ref>PMID:10386884</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10386884}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1b0a" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10386884 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10386884}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia coli K-12]]
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1B0A is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B0A OCA].
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[[Category: Large Structures]]
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[[Category: D'Ari L]]
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==Reference==
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[[Category: Dyer D]]
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<ref group="xtra">PMID:10386884</ref><references group="xtra"/>
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[[Category: Huang J-Y]]
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[[Category: Escherichia coli]]
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[[Category: Rabinowitz J]]
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[[Category: Ari, L D.]]
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[[Category: Shen BW]]
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[[Category: Dyer, D.]]
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[[Category: Stoddard BL]]
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[[Category: Huang, J Y.]]
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[[Category: Rabinowitz, J.]]
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[[Category: Shen, B W.]]
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[[Category: Stoddard, B L.]]
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[[Category: Bifunctional]]
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[[Category: Channeling]]
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[[Category: Cyclcohydrolase]]
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[[Category: Dehydrogenase]]
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[[Category: Folate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:57:21 2009''
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Current revision

5,10, METHYLENE-TETRAHYDROPHOLATE DEHYDROGENASE/CYCLOHYDROLASE FROM E COLI.

PDB ID 1b0a

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