1cfz

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{{Seed}}
 
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[[Image:1cfz.png|left|200px]]
 
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==HYDROGENASE MATURATING ENDOPEPTIDASE HYBD FROM E. COLI==
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The line below this paragraph, containing "STRUCTURE_1cfz", creates the "Structure Box" on the page.
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<StructureSection load='1cfz' size='340' side='right'caption='[[1cfz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1cfz]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CFZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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{{STRUCTURE_1cfz| PDB=1cfz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cfz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cfz OCA], [https://pdbe.org/1cfz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cfz RCSB], [https://www.ebi.ac.uk/pdbsum/1cfz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cfz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HYBD_ECOLI HYBD_ECOLI] Protease involved in the C-terminal processing of HybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of HybC.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cf/1cfz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cfz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The maturation of [NiFe] hydrogenases includes formation of the nickel metallocenter, proteolytic processing of the metal center carrying large subunit, and its assembling with other hydrogenase subunits. The hydrogenase maturating enzyme HYBD from Escherichia coli, a protease of molecular mass 17.5 kDa, specifically cleaves off a 15 amino acid peptide from the C terminus of the precursor of the large subunit of hydrogenase 2 in a nickel-dependent manner. Here we report the crystal structure of HYBD at 2.2 A resolution. It consists of a twisted five-stranded beta-sheet surrounded by four and three helices, respectively, on each side. A cadmium ion from the crystallization buffer binds to the proposed nickel-binding site and is penta-coordinated by Glu16, Asp62, His93, and a water molecule in a pseudo-tetragonal arrangement. HYBD is topologically related to members of the metzincins superfamily of zinc endoproteinases, sharing the central beta-sheet and three helices. In contrast to the metzincins, the metal-binding site of HYBD is localized at the C-terminal end of the beta-sheet. Three helical insertions unique to HYBD pack against one side of the sheet, build up the active site cleft, and provide His93 as ligand to the metal. From this structure, we derive molecular clues into how the protease HYBD is involved in the hydrogenase maturation process.
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===HYDROGENASE MATURATING ENDOPEPTIDASE HYBD FROM E. COLI===
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Crystal structure of the hydrogenase maturating endopeptidase HYBD from Escherichia coli.,Fritsche E, Paschos A, Beisel HG, Bock A, Huber R J Mol Biol. 1999 May 21;288(5):989-98. PMID:10331925<ref>PMID:10331925</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10331925}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1cfz" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10331925 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10331925}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1CFZ is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CFZ OCA].
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==Reference==
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<ref group="xtra">PMID:10331925</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Beisel, H G.]]
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[[Category: Large Structures]]
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[[Category: Boeck, A.]]
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[[Category: Beisel H-G]]
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[[Category: Fritsche, E.]]
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[[Category: Boeck A]]
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[[Category: Huber, R.]]
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[[Category: Fritsche E]]
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[[Category: Paschos, A.]]
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[[Category: Huber R]]
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[[Category: Hydrogenase]]
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[[Category: Paschos A]]
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[[Category: Maturation]]
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[[Category: Metzincin]]
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[[Category: Nickel]]
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[[Category: Protease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 18:37:47 2009''
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Current revision

HYDROGENASE MATURATING ENDOPEPTIDASE HYBD FROM E. COLI

PDB ID 1cfz

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