1j2n

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{{Seed}}
 
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[[Image:1j2n.png|left|200px]]
 
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==Solution structure of CPI-17(22-120) T38D==
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The line below this paragraph, containing "STRUCTURE_1j2n", creates the "Structure Box" on the page.
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<StructureSection load='1j2n' size='340' side='right'caption='[[1j2n]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1j2n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1J2N FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1j2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j2n OCA], [https://pdbe.org/1j2n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1j2n RCSB], [https://www.ebi.ac.uk/pdbsum/1j2n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1j2n ProSAT]</span></td></tr>
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{{STRUCTURE_1j2n| PDB=1j2n | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PP14A_PIG PP14A_PIG] Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.<ref>PMID:9237662</ref> <ref>PMID:8720121</ref> <ref>PMID:10924361</ref> <ref>PMID:10869555</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j2/1j2n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1j2n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present solution NMR structures for wild-type and mutated forms of CPI-17, a phosphoinhibitor for protein phosphatase 1. Phosphorylation of Thr38 of CPI-17 produces a &gt;1000-fold increase in inhibitory potency for myosin phosphatase. We compared the 1H-15N heteronuclear single quantum coherence spectroscopy (HSQC) chemical shifts of wild-type CPI-17, partially phosphorylated CPI-17 and CPI-17 with Thr38 replaced with Asp to introduce a negative charge. There was a switch in the protein conformation due to either Asp substitution or phosphorylation, so we determined the solution NMR structure of the CPI-17 T38D mutant as a model for the active (phospho-) conformation. The structures reveal a molecular switch in conformation that involves the rotation of two of the four helices in the four helix bundle. Despite this conformational switch, there was little increase in the inhibitory potency with T38D. We propose that for this inhibitor, a negative charge at residue 38 is sufficient to trigger an active conformation, but a phosphoryl group is required for full inhibitory potency against protein phosphatase-1.
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===Solution structure of CPI-17(22-120) T38D===
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Distinctive solution conformation of phosphatase inhibitor CPI-17 substituted with aspartate at the phosphorylation-site threonine residue.,Ohki S, Eto M, Shimizu M, Takada R, Brautigan DL, Kainosho M J Mol Biol. 2003 Mar 7;326(5):1539-47. PMID:12595264<ref>PMID:12595264</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12595264}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1j2n" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12595264 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12595264}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1J2N is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J2N OCA].
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==Reference==
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<ref group="xtra">PMID:12595264</ref><references group="xtra"/>
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Brautigan, D L.]]
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[[Category: Brautigan DL]]
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[[Category: Eto, M.]]
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[[Category: Eto M]]
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[[Category: Kainosho, M.]]
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[[Category: Kainosho M]]
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[[Category: Ohki, S.]]
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[[Category: Ohki S]]
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[[Category: Shimizu, M.]]
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[[Category: Shimizu M]]
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[[Category: Takada, R.]]
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[[Category: Takada R]]
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[[Category: Helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:03:05 2009''
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Current revision

Solution structure of CPI-17(22-120) T38D

PDB ID 1j2n

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