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2fez

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(New page: 200px<br /><applet load="2fez" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fez, resolution 2.00&Aring;" /> '''Mycobacterium tuberc...)
Current revision (09:23, 14 February 2024) (edit) (undo)
 
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[[Image:2fez.gif|left|200px]]<br /><applet load="2fez" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fez, resolution 2.00&Aring;" />
 
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'''Mycobacterium tuberculosis EmbR'''<br />
 
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==Overview==
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==Mycobacterium tuberculosis EmbR==
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Ser/Thr phosphorylation has emerged as a critical regulatory mechanism in, a number of bacteria, including Mycobacterium tuberculosis. This, problematic pathogen encodes 11 eukaryotic-like Ser/Thr kinases, yet few, substrates or signaling targets have been characterized. Here, we report, the structure of EmbR (2.0 A), a putative transcriptional regulator of key, arabinosyltransferases (EmbC, -A, and -B), and an endogenous substrate of, the Ser/Thr-kinase PknH. EmbR presents a unique domain architecture: the, N-terminal winged-helix DNA-binding domain forms an extensive interface, with the all-helical central bacterial transcriptional activation domain, and is positioned adjacent to the regulatory C-terminal, forkhead-associated (FHA) domain, which mediates binding to a, Thr-phosphorylated site in PknH. The structure in complex with a, phospho-peptide (1.9 A) reveals a conserved mode of phospho-threonine, recognition by the FHA domain and evidence for specific recognition of the, cognate kinase. The present structures suggest hypotheses as to how EmbR, might propagate the phospho-relay signal from its cognate kinase, while, serving as a template for the structurally uncharacterized Streptomyces, antibiotic regulatory protein family of transcription factors.
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<StructureSection load='2fez' size='340' side='right'caption='[[2fez]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fez]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FEZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FEZ FirstGlance]. <br>
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2FEZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FEZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fez FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fez OCA], [https://pdbe.org/2fez PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fez RCSB], [https://www.ebi.ac.uk/pdbsum/2fez PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fez ProSAT]</span></td></tr>
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==Reference==
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</table>
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Molecular structure of EmbR, a response element of Ser/Thr kinase signaling in Mycobacterium tuberculosis., Alderwick LJ, Molle V, Kremer L, Cozzone AJ, Dafforn TR, Besra GS, Futterer K, Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2558-63. Epub 2006 Feb 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16477027 16477027]
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== Function ==
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[[Category: Mycobacterium tuberculosis]]
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[https://www.uniprot.org/uniprot/EMBR_MYCTU EMBR_MYCTU] Positively regulates the transcription of the embCAB operon. Exhibits ATPase and GTPase activities.<ref>PMID:16817899</ref> <ref>PMID:16585755</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Alderwick, L.J.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Besra, G.S.]]
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Check<jmol>
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[[Category: Futterer, K.]]
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<jmolCheckbox>
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[[Category: transcriptional regulator; winged-helix; tetratricopeptide repeat; beta-sandwich;]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fe/2fez_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:31:27 2007''
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fez ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Alderwick LJ]]
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[[Category: Besra GS]]
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[[Category: Futterer K]]

Current revision

Mycobacterium tuberculosis EmbR

PDB ID 2fez

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