1epv

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{{Seed}}
 
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[[Image:1epv.png|left|200px]]
 
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==ALANINE RACEMASE WITH BOUND INHIBITOR DERIVED FROM D-CYCLOSERINE==
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The line below this paragraph, containing "STRUCTURE_1epv", creates the "Structure Box" on the page.
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<StructureSection load='1epv' size='340' side='right'caption='[[1epv]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1epv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EPV FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCS:D-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-N,O-CYCLOSERYLAMIDE'>DCS</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr>
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{{STRUCTURE_1epv| PDB=1epv | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1epv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1epv OCA], [https://pdbe.org/1epv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1epv RCSB], [https://www.ebi.ac.uk/pdbsum/1epv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1epv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALR_GEOSE ALR_GEOSE] Catalyzes the interconversion of L-alanine and D-alanine. Also weakly active on serine.<ref>PMID:10502689</ref> <ref>PMID:12203980</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ep/1epv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1epv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Alanine racemase (EC 5.1.1.1) catalyzes the interconversion of alanine enantiomers, and thus represents the first committed step involved in bacterial cell wall biosynthesis. Cycloserine acts as a suicide inhibitor of alanine racemase and as such, serves as an antimicrobial agent. The chemical means by which cycloserine inhibits alanine racemase is unknown. Through spectroscopic assays, we show here evidence of a pyridoxal derivative (arising from either isomer of cycloserine) saturated at the C4' carbon position. We additionally report the L- and D-cycloserine inactivated crystal structures of Bacillus stearothermophilus alanine racemase, which corroborates the spectroscopy via evidence of a 3-hydroxyisoxazole pyridoxamine derivative. Upon the basis of the kinetic and structural properties of both the L- and D-isomers of the inhibitor, we propose a mechanism of alanine racemase inactivation by cycloserine. This pathway involves an initial transamination step followed by tautomerization to form a stable aromatic adduct, a scheme similar to that seen in cycloserine inactivation of aminotransferases.
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===ALANINE RACEMASE WITH BOUND INHIBITOR DERIVED FROM D-CYCLOSERINE===
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A side reaction of alanine racemase: transamination of cycloserine.,Fenn TD, Stamper GF, Morollo AA, Ringe D Biochemistry. 2003 May 20;42(19):5775-83. PMID:12741835<ref>PMID:12741835</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1epv" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12741835}}, adds the Publication Abstract to the page
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*[[Alanine racemase 3D structures|Alanine racemase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12741835 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12741835}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1EPV is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EPV OCA].
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==Reference==
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<ref group="xtra">PMID:12741835</ref><references group="xtra"/>
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[[Category: Alanine racemase]]
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[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Fenn, T D.]]
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[[Category: Large Structures]]
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[[Category: Morollo, A A.]]
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[[Category: Fenn TD]]
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[[Category: Ringe, D.]]
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[[Category: Morollo AA]]
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[[Category: Stamper, G F.]]
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[[Category: Ringe D]]
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[[Category: Alpha-beta barrel]]
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[[Category: Stamper GF]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:43:12 2009''
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Current revision

ALANINE RACEMASE WITH BOUND INHIBITOR DERIVED FROM D-CYCLOSERINE

PDB ID 1epv

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