1m7x

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{{Seed}}
 
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[[Image:1m7x.png|left|200px]]
 
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==The X-ray Crystallographic Structure of Branching Enzyme==
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The line below this paragraph, containing "STRUCTURE_1m7x", creates the "Structure Box" on the page.
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<StructureSection load='1m7x' size='340' side='right'caption='[[1m7x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1m7x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M7X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m7x OCA], [https://pdbe.org/1m7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m7x RCSB], [https://www.ebi.ac.uk/pdbsum/1m7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m7x ProSAT]</span></td></tr>
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{{STRUCTURE_1m7x| PDB=1m7x | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLGB_ECOLI GLGB_ECOLI] Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m7/1m7x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m7x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Branching enzyme catalyzes the formation of alpha-1,6 branch points in either glycogen or starch. We report the 2.3-A crystal structure of glycogen branching enzyme from Escherichia coli. The enzyme consists of three major domains, an NH(2)-terminal seven-stranded beta-sandwich domain, a COOH-terminal domain, and a central alpha/beta-barrel domain containing the enzyme active site. While the central domain is similar to that of all the other amylase family enzymes, branching enzyme shares the structure of all three domains only with isoamylase. Oligosaccharide binding was modeled for branching enzyme using the enzyme-oligosaccharide complex structures of various alpha-amylases and cyclodextrin glucanotransferase and residues were implicated in oligosaccharide binding. While most of the oligosaccharides modeled well in the branching enzyme structure, an approximate 50 degrees rotation between two of the glucose units was required to avoid steric clashes with Trp(298) of branching enzyme. A similar rotation was observed in the mammalian alpha-amylase structure caused by an equivalent tryptophan residue in this structure. It appears that there are two binding modes for oligosaccharides in these structures depending on the identity and location of this aromatic residue.
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===The X-ray Crystallographic Structure of Branching Enzyme===
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The X-ray crystallographic structure of Escherichia coli branching enzyme.,Abad MC, Binderup K, Rios-Steiner J, Arni RK, Preiss J, Geiger JH J Biol Chem. 2002 Nov 1;277(44):42164-70. Epub 2002 Aug 23. PMID:12196524<ref>PMID:12196524</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12196524}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1m7x" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12196524 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12196524}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1M7X is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M7X OCA].
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==Reference==
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<ref group="xtra">PMID:12196524</ref><references group="xtra"/>
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[[Category: 1,4-alpha-glucan branching enzyme]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Abad, M C.]]
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[[Category: Large Structures]]
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[[Category: Arni, R K.]]
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[[Category: Abad MC]]
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[[Category: Binderup, K.]]
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[[Category: Arni RK]]
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[[Category: Geiger, J H.]]
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[[Category: Binderup K]]
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[[Category: Preiss, J.]]
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[[Category: Geiger JH]]
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[[Category: Rios-Steiner, J.]]
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[[Category: Preiss J]]
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[[Category: Alpha/beta barrel]]
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[[Category: Rios-Steiner J]]
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[[Category: Beta sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:44:22 2009''
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Current revision

The X-ray Crystallographic Structure of Branching Enzyme

PDB ID 1m7x

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