1wmz

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{{Seed}}
 
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[[Image:1wmz.png|left|200px]]
 
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==Crystal Structure of C-type Lectin CEL-I complexed with N-acetyl-D-galactosamine==
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The line below this paragraph, containing "STRUCTURE_1wmz", creates the "Structure Box" on the page.
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<StructureSection load='1wmz' size='340' side='right'caption='[[1wmz]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1wmz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Cucumaria_echinata Cucumaria echinata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WMZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NGA:N-ACETYL-D-GALACTOSAMINE'>NGA</scene></td></tr>
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{{STRUCTURE_1wmz| PDB=1wmz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wmz OCA], [https://pdbe.org/1wmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wmz RCSB], [https://www.ebi.ac.uk/pdbsum/1wmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wmz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7M462_CUCEC Q7M462_CUCEC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wm/1wmz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wmz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CEL-I is a C-type lectin, purified from the sea cucumber Cucumaria echinata, that shows a high specificity for N-acetylgalactosamine (GalNAc). We determined the crystal structures of CEL-I and its complex with GalNAc at 2.0 and 1.7 A resolution, respectively. CEL-I forms a disulfide-linked homodimer and contains two intramolecular disulfide bonds, although it lacks one intramolecular disulfide bond that is widely conserved among various C-type carbohydrate recognition domains (CRDs). Although the sequence similarity of CEL-I with other C-type CRDs is low, the overall folding of CEL-I was quite similar to those of other C-type CRDs. The structure of the complex with GalNAc revealed that the basic recognition mode of GalNAc was very similar to that for the GalNAc-binding mutant of the mannose-binding protein. However, the acetamido group of GalNAc appeared to be recognized more strongly by the combination of hydrogen bonds to Arg115 and van der Waals interaction with Gln70. Mutational analyses, in which Gln70 and/or Arg115 were replaced by alanine, confirmed that these residues contributed to GalNAc recognition in a cooperative manner.
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===Crystal Structure of C-type Lectin CEL-I complexed with N-acetyl-D-galactosamine===
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Characteristic recognition of N-acetylgalactosamine by an invertebrate C-type Lectin, CEL-I, revealed by X-ray crystallographic analysis.,Sugawara H, Kusunoki M, Kurisu G, Fujimoto T, Aoyagi H, Hatakeyama T J Biol Chem. 2004 Oct 22;279(43):45219-25. Epub 2004 Aug 19. PMID:15319425<ref>PMID:15319425</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15319425}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1wmz" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15319425 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15319425}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1WMZ is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Cucumaria_echinata Cucumaria echinata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WMZ OCA].
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==Reference==
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<ref group="xtra">PMID:15319425</ref><ref group="xtra">PMID:11752793</ref><references group="xtra"/>
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[[Category: Cucumaria echinata]]
[[Category: Cucumaria echinata]]
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[[Category: Aoyagi, H.]]
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[[Category: Large Structures]]
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[[Category: Fujimoto, T.]]
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[[Category: Aoyagi H]]
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[[Category: Hatakeyama, T.]]
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[[Category: Fujimoto T]]
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[[Category: Kurisu, G.]]
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[[Category: Hatakeyama T]]
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[[Category: Kusunoki, M.]]
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[[Category: Kurisu G]]
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[[Category: Sugawara, H.]]
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[[Category: Kusunoki M]]
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[[Category: C-type lectin]]
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[[Category: Sugawara H]]
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[[Category: Invertebrate]]
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[[Category: N-acetylgalactosamine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:49:09 2009''
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Current revision

Crystal Structure of C-type Lectin CEL-I complexed with N-acetyl-D-galactosamine

PDB ID 1wmz

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