2fkk

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(New page: 200px<br /><applet load="2fkk" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fkk, resolution 1.2&Aring;" /> '''Crystal structure of ...)
Current revision (09:24, 14 February 2024) (edit) (undo)
 
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[[Image:2fkk.gif|left|200px]]<br /><applet load="2fkk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fkk, resolution 1.2&Aring;" />
 
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'''Crystal structure of the C-terminal domain of the bacteriophage T4 gene product 10'''<br />
 
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==Overview==
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==Crystal structure of the C-terminal domain of the bacteriophage T4 gene product 10==
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The success of tailed bacteriophages to infect cells far exceeds that of, most other viruses on account of their specialized tail and associated, baseplate structures. The baseplate protein gene product (gp) 10 of, bacteriophage T4, whose structure was determined to 1.2 A resolution, was, fitted into the cryo-electron microscopy structures of the pre and, post-infection conformations of the virus. gp10 functions as a molecular, lever that rotates and extends the hinged short tail fibers to facilitate, cell attachment. The central folding motif of the gp10 trimer is similar, to that of the baseplate protein gp11 and to the receptor-binding domain, of the short tail fiber, gp12. The three proteins comprise the periphery, of the baseplate and interact with each other. The structural and, functional similarities of gp10, gp11, and gp12 and their sequential order, in the T4 genome suggest that they evolved separately, subsequent to gene, triplication from a common ancestor. Such events are usual in the, evolution of complex organelles from a common primordial molecule.
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<StructureSection load='2fkk' size='340' side='right'caption='[[2fkk]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fkk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FKK FirstGlance]. <br>
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2FKK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with BR, PO3, TRS and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FKK OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO3:PHOSPHITE+ION'>PO3</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fkk OCA], [https://pdbe.org/2fkk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fkk RCSB], [https://www.ebi.ac.uk/pdbsum/2fkk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fkk ProSAT]</span></td></tr>
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Evolution of bacteriophage tails: Structure of T4 gene product 10., Leiman PG, Shneider MM, Mesyanzhinov VV, Rossmann MG, J Mol Biol. 2006 May 5;358(3):912-21. Epub 2006 Mar 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16554069 16554069]
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</table>
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[[Category: Bacteriophage t4]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/BP10_BPT4 BP10_BPT4] Baseplate protein that is part of the baseplate wedge and that connects the short tail fibers to the baseplate (PubMed:16554069). During infection, the baseplate undergoes a conformational change from a dome-shaped to a star-shaped structure. At this point, gp10 rotates and acts as a lever that unfolds the short tail fibers, which then interact with host cell surface receptors. Involved in the tail assembly.<ref>PMID:16554069</ref> <ref>PMID:21129200</ref>
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[[Category: Leiman, P.G.]]
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== Evolutionary Conservation ==
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[[Category: Mesyanzhinov, V.V.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Rossmann, M.G.]]
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Check<jmol>
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[[Category: Shneider, M.M.]]
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<jmolCheckbox>
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[[Category: BR]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fk/2fkk_consurf.spt"</scriptWhenChecked>
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[[Category: EDO]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: PO3]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: TRS]]
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</jmolCheckbox>
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[[Category: bacteriophage t4; baseplate; tail; evolution; gp10; structural comparisons]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fkk ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:36:23 2007''
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia virus T4]]
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[[Category: Large Structures]]
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[[Category: Leiman PG]]
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[[Category: Mesyanzhinov VV]]
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[[Category: Rossmann MG]]
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[[Category: Shneider MM]]

Current revision

Crystal structure of the C-terminal domain of the bacteriophage T4 gene product 10

PDB ID 2fkk

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