2fmx

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(New page: 200px<br /><applet load="2fmx" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fmx, resolution 1.82&Aring;" /> '''An open conformation...)
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[[Image:2fmx.jpg|left|200px]]<br /><applet load="2fmx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fmx, resolution 1.82&Aring;" />
 
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'''An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)'''<br />
 
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==Overview==
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==An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)==
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Mg2+ is essential for guanosine triphosphatase activity and plays key, roles in guanine nucleotide binding and preserving the structural, integrity of GTP-binding proteins. To understand the structural basis for, Mg2+ function during the GDP/GTP exchange process, we determined the, crystal structure of Delta9-Sar1-GDP at low Mg2+ concentration at 1.8A., Two Sar1-GDP molecules in the crystal form a dimer with Mg2+ presenting, only in molecule B but not in molecule A. The absence of Mg2+ induces, significant conformational changes in the switch I region in molecule A, that shows similarities with those of Ha-Ras bound to Sos. The current, structure reveals an important regulatory role for Mg2+. We suggest that, guanine nucleotide exchange factor may utilize this feature to generate an, open conformation for GDP/GTP exchange. Furthermore, we propose a, mechanism for COPII assembly and disassembly in which dimerization of Sar1, plays an important role.
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<StructureSection load='2fmx' size='340' side='right'caption='[[2fmx]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2fmx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FMX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FMX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fmx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fmx OCA], [https://pdbe.org/2fmx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fmx RCSB], [https://www.ebi.ac.uk/pdbsum/2fmx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fmx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SAR1B_CRIGR SAR1B_CRIGR] Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.<ref>PMID:8138575</ref> <ref>PMID:11149932</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fm/2fmx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fmx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2FMX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with SO4, MG and GDP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FMX OCA].
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg2+., Rao Y, Bian C, Yuan C, Li Y, Chen L, Ye X, Huang Z, Huang M, Biochem Biophys Res Commun. 2006 Sep 29;348(3):908-15. Epub 2006 Aug 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16899220 16899220]
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__TOC__
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</StructureSection>
[[Category: Cricetulus griseus]]
[[Category: Cricetulus griseus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bian, C.]]
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[[Category: Bian C]]
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[[Category: Huang, M.]]
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[[Category: Huang M]]
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[[Category: Li, Y.]]
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[[Category: Li Y]]
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[[Category: Rao, Y.]]
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[[Category: Rao Y]]
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[[Category: Yuan, C.]]
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[[Category: Yuan C]]
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[[Category: GDP]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: cop ii assembly]]
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[[Category: crystal structure]]
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[[Category: dimerization]]
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[[Category: sar1]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:38:21 2007''
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Current revision

An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)

PDB ID 2fmx

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