2fnq

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(New page: 200px<br /><applet load="2fnq" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fnq, resolution 3.200&Aring;" /> '''Insights from the X...)
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[[Image:2fnq.gif|left|200px]]<br /><applet load="2fnq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fnq, resolution 3.200&Aring;" />
 
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'''Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality'''<br />
 
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==Overview==
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==Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality==
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Lipoxygenases (LOXs) catalyze the regio- and stereospecific dioxygenation, of polyunsaturated membrane-embedded fatty acids. We report here the 3.2 A, resolution structure of 8R-LOX from the Caribbean sea whip coral Plexaura, homomalla, a LOX isozyme with calcium dependence and the uncommon R chiral, stereospecificity. Structural and spectroscopic analyses demonstrated, calcium binding in a C2-like membrane-binding domain, illuminating the, function of similar amino acids in calcium-activated mammalian 5-LOX, the, key enzyme in the pathway to the pro-inflammatory leukotrienes. Mutation, of Ca(2+)-ligating amino acids in 8R-LOX resulted not only in a diminished, capacity to bind membranes, as monitored by fluorescence resonance energy, transfer, but also in an associated loss of Ca(2+)-regulated enzyme, activity. Moreover, a structural basis for R chiral specificity is also, revealed; creation of a small oxygen pocket next to Gly(428) (Ala in all, S-LOX isozymes) promoted C-8 oxygenation with R chirality on the activated, fatty acid substrate.
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<StructureSection load='2fnq' size='340' side='right'caption='[[2fnq]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fnq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plexaura_homomalla Plexaura homomalla]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1zq4 1zq4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FNQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FNQ FirstGlance]. <br>
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2FNQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Plexaura_homomalla Plexaura homomalla] with FE2 and CA as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1ZQ4. Active as [http://en.wikipedia.org/wiki/Arachidonate_8-lipoxygenase Arachidonate 8-lipoxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.40 1.13.11.40] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FNQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fnq OCA], [https://pdbe.org/2fnq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fnq RCSB], [https://www.ebi.ac.uk/pdbsum/2fnq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fnq ProSAT]</span></td></tr>
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Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via a C2-like domain and a structural basis of product chirality., Oldham ML, Brash AR, Newcomer ME, J Biol Chem. 2005 Nov 25;280(47):39545-52. Epub 2005 Sep 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16162493 16162493]
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</table>
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[[Category: Arachidonate 8-lipoxygenase]]
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== Function ==
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[https://www.uniprot.org/uniprot/AOSL_PLEHO AOSL_PLEHO] Bifunctional enzyme which is responsible for allene oxide biosynthesis via a two-step reaction which involves conversion of arachidonic acid to a 8R-hydroperoxide intermediate followed by conversion of the hydroperoxide to allene oxide.<ref>PMID:9302294</ref> <ref>PMID:10559269</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/2fnq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fnq ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Plexaura homomalla]]
[[Category: Plexaura homomalla]]
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[[Category: Single protein]]
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[[Category: Brash AR]]
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[[Category: Brash, A.R.]]
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[[Category: Newcomer ME]]
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[[Category: Newcomer, M.E.]]
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[[Category: Oldham ML]]
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[[Category: Oldham, M.L.]]
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[[Category: CA]]
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[[Category: FE2]]
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[[Category: beta-barrel]]
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[[Category: c2-like domain]]
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[[Category: eicosanoid]]
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[[Category: fatty acid]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:38:48 2007''
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Current revision

Insights from the X-ray crystal structure of coral 8R-lipoxygenase: calcium activation via A C2-like domain and a structural basis of product chirality

PDB ID 2fnq

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