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| - | [[Image:2fny.gif|left|200px]]<br /><applet load="2fny" size="450" color="white" frame="true" align="right" spinBox="true" | + | #REDIRECT [[3e47]] This PDB entry is obsolete and replaced by 3e47 |
| - | caption="2fny, resolution 3.00Å" />
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| - | '''Homobelactosin C bound to the yeast 20S proteasome'''<br />
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| - | ==Overview==
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| - | Most class I MHC ligands are generated from the vast majority of cellular, proteins by proteolysis within the ubiquitin-proteasome pathway and are, presented on the cell surface by MHC class I molecules. Here, we present, the crystallographic analysis of yeast 20S proteasome in complex with the, inhibitor homobelactosin C. The structure reveals a unique, inhibitor-binding mode and provides information about the composition of, proteasomal primed substrate-binding sites. IFN-gamma inducible, substitution of proteasomal constitutive subunits by immunosubunits, modulates characteristics of generated peptides, thus producing fragments, with higher preference for binding to MHC class I molecules. The, structural data for the proteasome:homobelactosin C complex provide an, explanation for involvement of immunosubunits in antigen generation and, open perspectives for rational design of ligands, inhibiting exclusively, constitutive proteasomes or immunoproteasomes.
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| - | ==About this Structure==
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| - | 2FNY is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with ESY as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Proteasome_endopeptidase_complex Proteasome endopeptidase complex], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.25.1 3.4.25.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FNY OCA].
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| - | ==Reference==
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| - | Inhibitor-binding mode of homobelactosin C to proteasomes: new insights into class I MHC ligand generation., Groll M, Larionov OV, Huber R, de Meijere A, Proc Natl Acad Sci U S A. 2006 Mar 21;103(12):4576-9. Epub 2006 Mar 13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16537370 16537370]
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| - | [[Category: Proteasome endopeptidase complex]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Saccharomyces cerevisiae]]
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| - | [[Category: Groll, M.]]
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| - | [[Category: ESY]]
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| - | [[Category: beta sandwich structure flanked by helices]]
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| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:38:56 2007''
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