1q9j

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{{Seed}}
 
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[[Image:1q9j.png|left|200px]]
 
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==Structure of polyketide synthase associated protein 5 from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_1q9j", creates the "Structure Box" on the page.
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<StructureSection load='1q9j' size='340' side='right'caption='[[1q9j]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1q9j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q9J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q9j OCA], [https://pdbe.org/1q9j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q9j RCSB], [https://www.ebi.ac.uk/pdbsum/1q9j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q9j ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1q9j TOPSAN]</span></td></tr>
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{{STRUCTURE_1q9j| PDB=1q9j | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PAPA5_MYCTU PAPA5_MYCTU] Catalyzes diesterification of phthiocerol and phthiodiolone with mycocerosic acids, the final step in the phthiocerol and phthiodiolone dimycocerosate esters (PDIM) synthesis. Can directly transfer the mycocerosate bound to the mycocerosic acid synthase (mas) onto the substrate alcohols. Is also able to catalyze acyl transfer using various nucleophiles as acceptors and several acyl-CoA thioesters as donors in vitro; preference is observed for saturated medium chain alcohols and long chain acyl-CoA thioesters.<ref>PMID:15070765</ref> <ref>PMID:15749014</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q9/1q9j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q9j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Polyketide-associated protein A5 (PapA5) is an acyltransferase that is involved in production of phthiocerol and phthiodiolone dimycocerosate esters, a class of virulence-enhancing lipids produced by Mycobacterium tuberculosis. Structural analysis of PapA5 at 2.75-A resolution reveals a two-domain structure that shares unexpected similarity to structures of chloramphenicol acetyltransferase, dihydrolipoyl transacetylase, carnitine acetyltransferase, and VibH, a non-ribosomal peptide synthesis condensation enzyme. The PapA5 active site includes conserved histidine and aspartic acid residues that are critical to PapA5 acyltransferase activity. PapA5 catalyzes acyl transfer reactions on model substrates that contain long aliphatic carbon chains, and two hydrophobic channels were observed linking the PapA5 surface to the active site with properties consistent with these biochemical activities and substrate preferences. An additional alpha helix not observed in other acyltransferase structures blocks the putative entrance into the PapA5 active site, indicating that conformational changes may be associated with PapA5 activity. PapA5 represents the first structure solved for a protein involved in polyketide synthesis in Mycobacteria.
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===Structure of polyketide synthase associated protein 5 from Mycobacterium tuberculosis===
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Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis.,Buglino J, Onwueme KC, Ferreras JA, Quadri LE, Lima CD J Biol Chem. 2004 Jul 16;279(29):30634-42. Epub 2004 May 3. PMID:15123643<ref>PMID:15123643</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1q9j" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15123643 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15123643}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1Q9J is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q9J OCA].
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==Reference==
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<ref group="xtra">PMID:15123643</ref><references group="xtra"/>
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Buglino, J.]]
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[[Category: Buglino J]]
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[[Category: Burley, S K.]]
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[[Category: Burley SK]]
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[[Category: Lima, C D.]]
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[[Category: Lima CD]]
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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[[Category: Onwueme KC]]
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[[Category: Onwueme, K C.]]
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[[Category: Quadri LE]]
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[[Category: Quadri, L E.]]
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[[Category: Conjugating enzyme papa5]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: New york structural genomix research consortium]]
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[[Category: Nysgxrc]]
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[[Category: Polyketide synthase associated protein]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 20:40:26 2009''
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Current revision

Structure of polyketide synthase associated protein 5 from Mycobacterium tuberculosis

PDB ID 1q9j

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