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2fsj

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(New page: 200px<br /><applet load="2fsj" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fsj, resolution 1.900&Aring;" /> '''Crystal structure o...)
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[[Image:2fsj.gif|left|200px]]<br /><applet load="2fsj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fsj, resolution 1.900&Aring;" />
 
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'''Crystal structure of Ta0583, an archaeal actin homolog, native data'''<br />
 
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==Overview==
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==Crystal structure of Ta0583, an archaeal actin homolog, native data==
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Prokaryotic homologs of the eukaryotic structural protein actin, such as, MreB and ParM, have been implicated in determination of bacterial cell, shape, and in the segregation of genomic and plasmid DNA. In contrast to, these bacterial actin homologs, little is known about the archaeal, counterparts. As a first step, we expressed a predicted actin homolog of, the thermophilic archaeon Thermoplasma acidophilum, Ta0583, and determined, its crystal structure at 2.1A resolution. Ta0583 is expressed as a soluble, protein in T.acidophilum and is an active ATPase at physiological, temperature. In vitro, Ta0583 forms sheets with spacings resembling the, crystal lattice, indicating an inherent propensity to form filamentous, structures. The fold of Ta0583 contains the core structure of actin and, clearly belongs to the actin/Hsp70 superfamily of ATPases. Ta0583 is, approximately equidistant from actin and MreB on the structural level, and, combines features from both eubacterial actin homologs, MreB and ParM. The, structure of Ta0583 co-crystallized with ADP indicates that the nucleotide, binds at the interface between the subdomains of Ta0583 in a manner, similar to that of actin. However, the conformation of the nucleotide, observed in complex with Ta0583 clearly differs from that in complex with, actin, but closely resembles the conformation of ParM-bound nucleotide. On, the basis of sequence and structural homology, we suggest that Ta0583, derives from a ParM-like actin homolog that was once encoded by a plasmid, and was transferred into a common ancestor of Thermoplasma and, Ferroplasma. Intriguingly, both genera are characterized by the lack of a, cell wall, and therefore Ta0583 could have a function in cellular, organization.
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<StructureSection load='2fsj' size='340' side='right'caption='[[2fsj]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fsj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum_DSM_1728 Thermoplasma acidophilum DSM 1728]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FSJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FSJ FirstGlance]. <br>
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2FSJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FSJ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fsj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fsj OCA], [https://pdbe.org/2fsj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fsj RCSB], [https://www.ebi.ac.uk/pdbsum/2fsj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fsj ProSAT]</span></td></tr>
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Crystal structure of an archaeal actin homolog., Roeben A, Kofler C, Nagy I, Nickell S, Hartl FU, Bracher A, J Mol Biol. 2006 Apr 21;358(1):145-56. Epub 2006 Feb 9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16500678 16500678]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Thermoplasma acidophilum]]
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[https://www.uniprot.org/uniprot/ACTH_THEAC ACTH_THEAC] Polymerizes into bundles of filaments, forming a helix with a filament width of 5.5 nm and an axial repeating unit of 5.5 nm. Polymerization of Ta0583 requires NTP and is optimal with ATP, but GTP, UTP, CTP, and even the deoxy form of NTP can also support the polymerization reaction. Nucleoside diphosphate or AMP-PNP does not support polymerization.<ref>PMID:16500678</ref> <ref>PMID:17189356</ref>
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[[Category: Bracher, A.]]
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== Evolutionary Conservation ==
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[[Category: Hartl, F.Ulrich.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Kofler, C.]]
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Check<jmol>
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[[Category: Nagy, I.]]
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<jmolCheckbox>
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[[Category: Nickell, S.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fs/2fsj_consurf.spt"</scriptWhenChecked>
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[[Category: Roeben, A.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: GOL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: actin homologs]]
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</jmolCheckbox>
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[[Category: archaea]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fsj ConSurf].
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[[Category: atpase]]
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<div style="clear:both"></div>
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[[Category: mreb]]
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== References ==
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[[Category: parm]]
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<references/>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:44:30 2007''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermoplasma acidophilum DSM 1728]]
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[[Category: Bracher A]]
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[[Category: Kofler C]]
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[[Category: Nagy I]]
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[[Category: Nickell S]]
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[[Category: Roeben A]]
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[[Category: Ulrich Hartl F]]

Current revision

Crystal structure of Ta0583, an archaeal actin homolog, native data

PDB ID 2fsj

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