2fte

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(New page: 200px<br /><applet load="2fte" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fte" /> '''Bacteriophage HK97 Expansion Intermediate IV...)
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[[Image:2fte.gif|left|200px]]<br /><applet load="2fte" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2fte" />
 
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'''Bacteriophage HK97 Expansion Intermediate IV'''<br />
 
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==Overview==
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==Bacteriophage HK97 Expansion Intermediate IV==
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Maturation of the bacteriophage HK97 capsid from a precursor (Prohead II), to the mature state (Head II) involves a 60 A radial expansion. The mature, particle is formed by 420 copies of the major capsid protein organized on, a T = 7 laevo lattice with each subunit covalently crosslinked to two, neighbors. Well-characterized pH 4 expansion intermediates make HK97, valuable for investigating quaternary structural dynamics. Here, we use, X-ray crystallography and cryo-EM to demonstrate that in the final, transition in maturation (requiring neutral pH), pentons in Expansion, Intermediate IV (EI-IV) reversibly sample 14 A translations and 6 degrees, rotations relative to a fixed hexon lattice. The limit of this trajectory, corresponds to the Head II conformation that is secured at this extent, only by the formation of the final class of covalent crosslinks. Mutants, that cannot crosslink or EI-IV particles that have been rendered incapable, of forming the final crosslink remain in the EI-IV state.
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<SX load='2fte' size='340' side='right' viewer='molstar' caption='[[2fte]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2fte]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FTE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FTE FirstGlance]. <br>
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2FTE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk620 Enterobacteria phage hk620]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2FTE OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fte FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fte OCA], [https://pdbe.org/2fte PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fte RCSB], [https://www.ebi.ac.uk/pdbsum/2fte PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fte ProSAT]</span></td></tr>
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==Reference==
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</table>
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Capsid conformational sampling in HK97 maturation visualized by X-ray crystallography and cryo-EM., Gan L, Speir JA, Conway JF, Lander G, Cheng N, Firek BA, Hendrix RW, Duda RL, Liljas L, Johnson JE, Structure. 2006 Nov;14(11):1655-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17098191 17098191]
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== Function ==
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[[Category: Enterobacteria phage hk620]]
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[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Cheng, N.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Conway, J.F.]]
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Check<jmol>
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[[Category: Duda, R.L.]]
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<jmolCheckbox>
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[[Category: Firek, B.A.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/2fte_consurf.spt"</scriptWhenChecked>
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[[Category: Gan, L.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Hendrix, R.W.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Johnson, J.E.]]
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</jmolCheckbox>
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[[Category: Lander, G.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fte ConSurf].
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[[Category: Liljas, L.]]
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<div style="clear:both"></div>
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[[Category: Speir, J.A.]]
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== References ==
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[[Category: bacteriophage]]
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<references/>
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[[Category: capsid protein]]
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__TOC__
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[[Category: expansion intermediate]]
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</SX>
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[[Category: hk97]]
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[[Category: Escherichia virus HK97]]
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[[Category: Large Structures]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:45:35 2007''
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[[Category: Cheng N]]
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[[Category: Conway JF]]
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[[Category: Duda RL]]
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[[Category: Firek BA]]
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[[Category: Gan L]]
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[[Category: Hendrix RW]]
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[[Category: Johnson JE]]
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[[Category: Lander G]]
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[[Category: Liljas L]]
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[[Category: Speir JA]]

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Bacteriophage HK97 Expansion Intermediate IV

2fte

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