1ymh

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{{Seed}}
 
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[[Image:1ymh.png|left|200px]]
 
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==anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W==
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The line below this paragraph, containing "STRUCTURE_1ymh", creates the "Structure Box" on the page.
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<StructureSection load='1ymh' size='340' side='right'caption='[[1ymh]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ymh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Finegoldia_magna_ATCC_29328 Finegoldia magna ATCC 29328] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YMH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ymh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ymh OCA], [https://pdbe.org/1ymh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ymh RCSB], [https://www.ebi.ac.uk/pdbsum/1ymh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ymh ProSAT]</span></td></tr>
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{{STRUCTURE_1ymh| PDB=1ymh | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q51918_FINMA Q51918_FINMA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ym/1ymh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ymh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein L from Peptostreptococcus magnus (PpL) is a multidomain protein composed of four or five immunoglobulin-binding domains that target the kappa light chain of a large repertoire of human and murine antibodies. Thus, a single domain of this protein can be used to aid the crystallization of Fab, free or complexed to their antigen when it is not possible to obtain crystals without it. Each wild-type PpL domain has two light-chain binding sites that target the same region of the light chain and can thus bring together two Fab-antigen complexes within the crystal lattice. In this context the small PpL domain is sandwiched between two Fab and cannot participate in crystal contacts, thus mutants are unlikely to increase the chances of crystallizing a particular complex. However, it is possible to design mutants that can bind at only one site by making use of the crystal structures obtained so far. Such mutants will have a free surface that can participate in crystal contacts and that can be modified to improve its crystal contact-forming properties. Here, a comparison of two single-site mutants that differ at three different positions is reported. In both mutants two different tryptophan residues participate in crystal-packing interactions, suggesting that this residue may be particularly interesting for enhancing crystal-contact formation.
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===anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W===
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Comparison of the crystallization and crystal packing of two Fab single-site mutant protein L complexes.,Granata V, Housden NG, Harrison S, Jolivet-Reynaud C, Gore MG, Stura EA Acta Crystallogr D Biol Crystallogr. 2005 Jun;61(Pt 6):750-4. Epub 2005, May 26. PMID:15930633<ref>PMID:15930633</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ymh" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15930633}}, adds the Publication Abstract to the page
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15930633 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15930633}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Finegoldia magna ATCC 29328]]
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1YMH is a 6 chains structure of sequences from [http://en.wikipedia.org/wiki/Finegoldia_magna Finegoldia magna] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YMH OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:15930633</ref><references group="xtra"/>
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[[Category: Finegoldia magna]]
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Gore, M G.]]
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[[Category: Gore MG]]
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[[Category: Granata, V.]]
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[[Category: Granata V]]
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[[Category: Harrison, S.]]
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[[Category: Harrison S]]
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[[Category: Housden, N G.]]
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[[Category: Housden NG]]
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[[Category: Jolivet-Reynaud, C.]]
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[[Category: Jolivet-Reynaud C]]
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[[Category: Stura, E A.]]
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[[Category: Stura EA]]
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[[Category: Engineering of crystal contact]]
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[[Category: Ppl-fab complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:22:36 2009''
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Current revision

anti-HCV Fab 19D9D6 complexed with protein L (PpL) mutant A66W

PDB ID 1ymh

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