2err

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:55, 9 March 2022) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2err.png|left|200px]]
 
-
<!--
+
==NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU==
-
The line below this paragraph, containing "STRUCTURE_2err", creates the "Structure Box" on the page.
+
<StructureSection load='2err' size='340' side='right'caption='[[2err]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2err]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ERR FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A2BP1, A2BP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2err FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2err OCA], [https://pdbe.org/2err PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2err RCSB], [https://www.ebi.ac.uk/pdbsum/2err PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2err ProSAT]</span></td></tr>
-
{{STRUCTURE_2err| PDB=2err | SCENE= }}
+
</table>
 +
== Function ==
 +
[[https://www.uniprot.org/uniprot/RFOX1_HUMAN RFOX1_HUMAN]] RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.<ref>PMID:16537540</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/er/2err_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2err ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The Fox-1 protein regulates alternative splicing of tissue-specific exons by binding to GCAUG elements. Here, we report the solution structure of the Fox-1 RNA binding domain (RBD) in complex with UGCAUGU. The last three nucleotides, UGU, are recognized in a canonical way by the four-stranded beta-sheet of the RBD. In contrast, the first four nucleotides, UGCA, are bound by two loops of the protein in an unprecedented manner. Nucleotides U1, G2, and C3 are wrapped around a single phenylalanine, while G2 and A4 form a base-pair. This novel RNA binding site is independent from the beta-sheet binding interface. Surface plasmon resonance analyses were used to quantify the energetic contributions of electrostatic and hydrogen bond interactions to complex formation and support our structural findings. These results demonstrate the unusual molecular mechanism of sequence-specific RNA recognition by Fox-1, which is exceptional in its high affinity for a defined but short sequence element.
-
===NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU===
+
Molecular basis of RNA recognition by the human alternative splicing factor Fox-1.,Auweter SD, Fasan R, Reymond L, Underwood JG, Black DL, Pitsch S, Allain FH EMBO J. 2006 Jan 11;25(1):163-73. Epub 2005 Dec 15. PMID:16362037<ref>PMID:16362037</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16362037}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2err" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16362037 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16362037}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Human]]
-
2ERR is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ERR OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Allain, F H]]
-
==Reference==
+
[[Category: Auweter, S D]]
-
<ref group="xtra">PMID:16362037</ref><references group="xtra"/>
+
-
[[Category: Homo sapiens]]
+
-
[[Category: Allain, F H.]]
+
-
[[Category: Auweter, S D.]]
+
[[Category: Protein-rna complex]]
[[Category: Protein-rna complex]]
-
 
+
[[Category: Rna binding protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:59:46 2009''
+

Current revision

NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU

PDB ID 2err

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2err"
Personal tools