2azk

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{{Seed}}
 
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[[Image:2azk.png|left|200px]]
 
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==Crystal structure for the mutant W136E of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase==
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The line below this paragraph, containing "STRUCTURE_2azk", creates the "Structure Box" on the page.
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<StructureSection load='2azk' size='340' side='right'caption='[[2azk]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2azk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2AZK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2azk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2azk OCA], [https://pdbe.org/2azk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2azk RCSB], [https://www.ebi.ac.uk/pdbsum/2azk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2azk ProSAT]</span></td></tr>
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{{STRUCTURE_2azk| PDB=2azk | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HEXPP_SACS2 HEXPP_SACS2] Catalyzes consecutive E-type condensation of two isopentenyl pyrophosphate (IPP) molecules with an allylic substrate such as geranylgeranyl diphosphate (GGPP), farnesyl diphosphate (FPP) or geranyl diphosphate (GPP) to yield the medium-chain product trans-C30-hexaprenyl pyrophosphate (HexPP). GGPP is the physiological substrate.<ref>PMID:11790729</ref> <ref>PMID:16291686</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/az/2azk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2azk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Hexaprenyl pyrophosphate synthase (HexPPs) from Sulfolobus solfataricus catalyzes the synthesis of trans-C(30)-hexaprenyl pyrophosphate (HexPP) by reacting two isopentenyl pyrophosphate molecules with one geranylgeranyl pyrophosphate. The crystal structure of the homodimeric C(30)-HexPPs resembles those of other trans-prenyltransferases, including farnesyl pyrophosphate synthase (FPPs) and octaprenyl pyrophosphate synthase (OPPs). In both subunits, 10 core helices are arranged about a central active site cavity. Leu164 in the middle of the cavity controls the product chain length. Two protein conformers are observed in the S. solfataricus HexPPs structure, and the major difference between them occurs in the flexible region of residues 84 to 100. Several helices (alphaI, alphaJ, alphaK, and part of alphaH) and the associated loops have high-temperature factors in one monomer, which may be related to the domain motion that controls the entrance to the active site. Different side chain conformations of Trp136 in two HexPPs subunits result in weaker hydrophobic interactions at the dimer interface, in contrast to the symmetric pi-pi stacking interactions of aromatic side chains found in FPPs and OPPs. Finally, the three-conformer switched model may explain the catalytic process for HexPPs.
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===Crystal structure for the mutant W136E of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase===
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Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures.,Sun HY, Ko TP, Kuo CJ, Guo RT, Chou CC, Liang PH, Wang AH J Bacteriol. 2005 Dec;187(23):8137-48. PMID:16291686<ref>PMID:16291686</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2azk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16291686}}, adds the Publication Abstract to the page
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*[[Geranylgeranyl pyrophosphate synthase 3D structures|Geranylgeranyl pyrophosphate synthase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16291686 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16291686}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2AZK is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AZK OCA].
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[[Category: Saccharolobus solfataricus]]
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[[Category: Chou CC]]
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==Reference==
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[[Category: Guo RT]]
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<ref group="xtra">PMID:16291686</ref><references group="xtra"/>
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[[Category: Ko TP]]
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[[Category: Dimethylallyltranstransferase]]
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[[Category: Kuo CJ]]
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[[Category: Sulfolobus solfataricus]]
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[[Category: Liang PH]]
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[[Category: Chou, C C.]]
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[[Category: Sun HY]]
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[[Category: Guo, R T.]]
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[[Category: Wang AHJ]]
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[[Category: Ko, T P.]]
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[[Category: Kuo, C J.]]
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[[Category: Liang, P H.]]
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[[Category: Sun, H Y.]]
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[[Category: Wang, A H.J.]]
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[[Category: Hexpp]]
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[[Category: Isoprenyl pyrophosphate synthase]]
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[[Category: Trans-prenyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 22:04:12 2009''
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Current revision

Crystal structure for the mutant W136E of Sulfolobus solfataricus hexaprenyl pyrophosphate synthase

PDB ID 2azk

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