1fb9

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{{Seed}}
 
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[[Image:1fb9.png|left|200px]]
 
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==EFFECTS OF S-SULFONATION ON THE SOLUTION STRUCTURE OF SALMON CALCITONIN==
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The line below this paragraph, containing "STRUCTURE_1fb9", creates the "Structure Box" on the page.
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<StructureSection load='1fb9' size='340' side='right'caption='[[1fb9]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1fb9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncorhynchus_gorbuscha Oncorhynchus gorbuscha]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FB9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSU:CYSTEINE-S-SULFONIC+ACID'>CSU</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
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{{STRUCTURE_1fb9| PDB=1fb9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fb9 OCA], [https://pdbe.org/1fb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fb9 RCSB], [https://www.ebi.ac.uk/pdbsum/1fb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fb9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8QG84_ONCGO Q8QG84_ONCGO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fb/1fb9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fb9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Salmon calcitonin S-sulfonated analog (abbreviated as [S-SO(3)(-)]rsCT) was prepared by introducing two sulfonic groups into the side chains of Cys1 and Cys7 of recombinant salmon calcitonin. The hypocalcemic potency of this open-chain analog is 5500IU/mg, which is about 30% higher than that (4500IU/mg) of the wild type. The solution conformation of [S-SO(3)(-)]rsCT was studied in aqueous trifluoroethanol solution by CD, 2D-NMR spectroscopy, and distance geometry calculations. In the mixture of 60% TFE and 40% water, the peptide assumes an amphipathic alpha-helix in the region of residues 4-22, which is one turn longer than that of the native sCT. The structural feature analysis of the peptide revealed the presence of hydrophobic surface composed of five hydrophobic side chains of residues Leu4, Leu9, Leu12, Leu16, and Leu19, and a network of salt-bridges that consisted of a tetrad of oppositely charged side chains (Cys7-SO(3)(-)-Lys11(+)-Glu15(-)-Lys18(+)). The multiple salt bridges resulted in the stabilization of the longer amphipathic alpha-helix. Meanwhile, the higher hypocalcemic potency of the peptide could be attributed to the array of hydrophobic side chains of five leucine residues of the amphipathic alpha-helix.
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===EFFECTS OF S-SULFONATION ON THE SOLUTION STRUCTURE OF SALMON CALCITONIN===
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Solution structure and biological activity of recombinant salmon calcitonin S-sulfonated analog.,Wang Y, Dou H, Cao C, Zhang N, Ma J, Mao J, Wu H Biochem Biophys Res Commun. 2003 Jun 27;306(2):582-9. PMID:12804605<ref>PMID:12804605</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12804605}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1fb9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12804605 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12804605}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1FB9 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_gorbuscha Oncorhynchus gorbuscha]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FB9 OCA].
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==Reference==
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<ref group="xtra">PMID:12804605</ref><references group="xtra"/>
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[[Category: Oncorhynchus gorbuscha]]
[[Category: Oncorhynchus gorbuscha]]
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[[Category: Dou, H.]]
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[[Category: Dou H]]
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[[Category: Mao, J.]]
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[[Category: Mao J]]
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[[Category: Wang, Y.]]
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[[Category: Wang Y]]
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[[Category: Wu, H.]]
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[[Category: Wu H]]
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[[Category: Alpha helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 22:07:44 2009''
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Current revision

EFFECTS OF S-SULFONATION ON THE SOLUTION STRUCTURE OF SALMON CALCITONIN

PDB ID 1fb9

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