2g4m

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(New page: 200px<br /><applet load="2g4m" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g4m, resolution 1.80&Aring;" /> '''Insulin collected at...)
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[[Image:2g4m.jpg|left|200px]]<br /><applet load="2g4m" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2g4m, resolution 1.80&Aring;" />
 
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'''Insulin collected at 2.0 A wavelength'''<br />
 
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==Overview==
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==Insulin collected at 2.0 A wavelength==
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23 different crystal forms of 19 different biological macromolecules were, examined with respect to their anomalously scattering substructures using, diffraction data collected at a wavelength of 2.0 A (6.2 keV). In more, than 90% of the cases the substructure was found to contain more than just, the protein S atoms. The data presented suggest that chloride, sulfate, phosphate or metal ions from the buffer or even from the purification, protocol are frequently bound to the protein molecule and that these ions, are often overlooked, especially if they are not bound at full occupancy., Thus, in order to fully describe the macromolecule under study, it seems, desirable that any structure determination be complemented with a, long-wavelength data set.
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<StructureSection load='2g4m' size='340' side='right'caption='[[2g4m]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2g4m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G4M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G4M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g4m OCA], [https://pdbe.org/2g4m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g4m RCSB], [https://www.ebi.ac.uk/pdbsum/2g4m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g4m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/INS_PIG INS_PIG] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g4/2g4m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g4m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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23 different crystal forms of 19 different biological macromolecules were examined with respect to their anomalously scattering substructures using diffraction data collected at a wavelength of 2.0 A (6.2 keV). In more than 90% of the cases the substructure was found to contain more than just the protein S atoms. The data presented suggest that chloride, sulfate, phosphate or metal ions from the buffer or even from the purification protocol are frequently bound to the protein molecule and that these ions are often overlooked, especially if they are not bound at full occupancy. Thus, in order to fully describe the macromolecule under study, it seems desirable that any structure determination be complemented with a long-wavelength data set.
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==About this Structure==
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On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths.,Mueller-Dieckmann C, Panjikar S, Schmidt A, Mueller S, Kuper J, Geerlof A, Wilmanns M, Singh RK, Tucker PA, Weiss MS Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):366-80. Epub 2007, Feb 21. PMID:17327674<ref>PMID:17327674</ref>
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2G4M is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2G4M OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths., Mueller-Dieckmann C, Panjikar S, Schmidt A, Mueller S, Kuper J, Geerlof A, Wilmanns M, Singh RK, Tucker PA, Weiss MS, Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):366-80. Epub 2007, Feb 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17327674 17327674]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 2g4m" style="background-color:#fffaf0;"></div>
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[[Category: Sus scrofa]]
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[[Category: Mueller-Dieckmann, C.]]
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[[Category: Weiss, M.S.]]
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[[Category: insulin at 2.0 a wavelength]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 10:56:41 2007''
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==See Also==
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*[[Insulin 3D Structures|Insulin 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Sus scrofa]]
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[[Category: Mueller-Dieckmann C]]
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[[Category: Weiss MS]]

Current revision

Insulin collected at 2.0 A wavelength

PDB ID 2g4m

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