1a99

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:15, 23 October 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1a99.png|left|200px]]
 
-
<!--
+
==PUTRESCINE RECEPTOR (POTF) FROM E. COLI==
-
The line below this paragraph, containing "STRUCTURE_1a99", creates the "Structure Box" on the page.
+
<StructureSection load='1a99' size='340' side='right'caption='[[1a99]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1a99]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A99 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene></td></tr>
-
{{STRUCTURE_1a99| PDB=1a99 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a99 OCA], [https://pdbe.org/1a99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a99 RCSB], [https://www.ebi.ac.uk/pdbsum/1a99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a99 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/POTF_ECOLI POTF_ECOLI] Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a9/1a99_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a99 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
PotF protein is a periplasmic substrate-binding protein of the putrescine transport system in Escherichia coli. We have determined the crystal structure of PotF protein in complex with the substrate at 2.3-A resolution. The PotF molecule has dimensions of 54 x 42 x 30 A and consists of two similar globular domains. The PotF structure is reminiscent of other periplasmic receptors with a highest structural homology to another polyamine-binding protein, PotD. Putrescine is tightly bound in the deep cleft between the two domains of PotF through 12 hydrogen bonds and 36 van der Waals interactions. The comparison of the PotF structure with that of PotD provides the insight into the differences in the specificity between the two proteins. The PotF structure, in combination with the mutational analysis, revealed the residues crucial for putrescine binding (Trp-37, Ser-85, Glu-185, Trp-244, Asp-247, and Asp-278) and the importance of water molecules for putrescine recognition.
-
===PUTRESCINE RECEPTOR (POTF) FROM E. COLI===
+
Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity.,Vassylyev DG, Tomitori H, Kashiwagi K, Morikawa K, Igarashi K J Biol Chem. 1998 Jul 10;273(28):17604-9. PMID:9651355<ref>PMID:9651355</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_9651355}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1a99" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 9651355 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_9651355}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1A99 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A99 OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:9651355</ref><references group="xtra"/>
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Igarashi, K.]]
+
[[Category: Large Structures]]
-
[[Category: Kashiwagi, K.]]
+
[[Category: Igarashi K]]
-
[[Category: Morikawa, K.]]
+
[[Category: Kashiwagi K]]
-
[[Category: Tomitori, H.]]
+
[[Category: Morikawa K]]
-
[[Category: Vassylyev, D G.]]
+
[[Category: Tomitori H]]
-
[[Category: Binding protein]]
+
[[Category: Vassylyev DG]]
-
[[Category: Transport]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 22:40:52 2009''
+

Current revision

PUTRESCINE RECEPTOR (POTF) FROM E. COLI

PDB ID 1a99

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1a99"
Personal tools