1wbd

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{{Seed}}
 
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[[Image:1wbd.png|left|200px]]
 
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==Crystal structure of E. coli DNA mismatch repair enzyme MutS, E38Q mutant, in complex with a G.T mismatch==
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The line below this paragraph, containing "STRUCTURE_1wbd", creates the "Structure Box" on the page.
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<StructureSection load='1wbd' size='340' side='right'caption='[[1wbd]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1wbd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WBD FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1wbd| PDB=1wbd | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wbd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbd OCA], [https://pdbe.org/1wbd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wbd RCSB], [https://www.ebi.ac.uk/pdbsum/1wbd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wbd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MUTS_ECOLI MUTS_ECOLI] This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wb/1wbd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wbd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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MutS plays a critical role in DNA mismatch repair in Escherichia coli by binding to mismatches and initiating repair in an ATP-dependent manner. Mutational analysis of a highly conserved glutamate, Glu38, has revealed its role in mismatch recognition by enabling MutS to discriminate between homoduplex and mismatched DNA. Crystal structures of MutS have shown that Glu38 forms a hydrogen bond to one of the mismatched bases. In this study, we have analyzed the crystal structures, DNA binding and the response to ATP binding of three Glu38 mutants. While confirming the role of the negative charge in initial discrimination, we show that in vivo mismatch repair can proceed even when discrimination is low. We demonstrate that the formation of a hydrogen bond by residue 38 to the mismatched base authorizes repair by inducing intramolecular signaling, which results in the inhibition of rapid hydrolysis of distally bound ATP. This allows formation of the stable MutS-ATP-DNA clamp, a key intermediate in triggering downstream repair events.
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===CRYSTAL STRUCTURE OF E. COLI DNA MISMATCH REPAIR ENZYME MUTS, E38Q MUTANT, IN COMPLEX WITH A G.T MISMATCH===
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Dual role of MutS glutamate 38 in DNA mismatch discrimination and in the authorization of repair.,Lebbink JH, Georgijevic D, Natrajan G, Fish A, Winterwerp HH, Sixma TK, de Wind N EMBO J. 2006 Jan 25;25(2):409-19. Epub 2006 Jan 12. PMID:16407973<ref>PMID:16407973</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1wbd" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16407973}}, adds the Publication Abstract to the page
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*[[DNA mismatch repair protein 3D structures|DNA mismatch repair protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16407973 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16407973}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1WBD is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WBD OCA].
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==Reference==
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<ref group="xtra">PMID:16407973</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Georgijevic, D.]]
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[[Category: Large Structures]]
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[[Category: Lebbink, J H.G.]]
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[[Category: Georgijevic D]]
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[[Category: Natrajan, G.]]
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[[Category: Lebbink JHG]]
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[[Category: Sixma, T K.]]
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[[Category: Natrajan G]]
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[[Category: Wind, N De.]]
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[[Category: Sixma TK]]
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[[Category: Winterwerp, H H.K.]]
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[[Category: Winterwerp HHK]]
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[[Category: Atp-binding]]
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[[Category: De Wind N]]
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[[Category: Complete proteome]]
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[[Category: Dna binding]]
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[[Category: Dna repair]]
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[[Category: Mismatch recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 23:53:29 2009''
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Current revision

Crystal structure of E. coli DNA mismatch repair enzyme MutS, E38Q mutant, in complex with a G.T mismatch

PDB ID 1wbd

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