2g7o

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(New page: 200px<br /><applet load="2g7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="2g7o, resolution 1.400&Aring;" /> '''Protonation-mediate...)
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[[Image:2g7o.gif|left|200px]]<br /><applet load="2g7o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2g7o, resolution 1.400&Aring;" />
 
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'''Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM'''<br />
 
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==Overview==
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==Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM==
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TraM is essential for F plasmid-mediated bacterial conjugation, where it, binds to the plasmid DNA near the origin of transfer, and recognizes a, component of the transmembrane DNA transfer complex, TraD. Here we report, the 1.40 A crystal structure of the TraM core tetramer (TraM58-127)., TraM58-127 is a compact eight-helical bundle, in which the N-terminal, helices from each protomer interact to form a central, parallel, four-stranded coiled-coil, whereas each C-terminal helix packs in an, antiparallel arrangement around the outside of the structure. Four, protonated glutamic acid residues (Glu88) are packed in a hydrogen-bonded, arrangement within the central four-helix bundle. Mutational and, biophysical analyses indicate that this protonated state is in equilibrium, with a deprotonated tetrameric form characterized by a lower helical, content at physiological pH and temperature. Comparison of TraM to its, Glu88 mutants predicted to stabilize the helical structure suggests that, the protonated state is the active form for binding TraD in conjugation.
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<StructureSection load='2g7o' size='340' side='right'caption='[[2g7o]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2g7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2G7O FirstGlance]. <br>
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2G7O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2G7O OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2g7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g7o OCA], [https://pdbe.org/2g7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2g7o RCSB], [https://www.ebi.ac.uk/pdbsum/2g7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2g7o ProSAT]</span></td></tr>
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==Reference==
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</table>
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Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM., Lu J, Edwards RA, Wong JJ, Manchak J, Scott PG, Frost LS, Glover JN, EMBO J. 2006 Jun 21;25(12):2930-9. Epub 2006 May 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16710295 16710295]
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== Function ==
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[https://www.uniprot.org/uniprot/TRAM1_ECOLI TRAM1_ECOLI] Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Cooperatively binds 3 regions in the F plasmid oriT locus; 2 are required for autoregulation while the other is required for plasmid transfer. Bends oriT DNA less than 50 degrees. Plasmid specificity is conferred by the TraD-TraM pair.<ref>PMID:1479887</ref> <ref>PMID:8736534</ref> <ref>PMID:11875064</ref> <ref>PMID:17238924</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/g7/2g7o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2g7o ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Edwards, R.A.]]
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[[Category: Edwards RA]]
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[[Category: Frost, L.S.]]
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[[Category: Frost LS]]
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[[Category: Glover, J.N.]]
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[[Category: Glover JN]]
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[[Category: Lu, J.]]
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[[Category: Lu J]]
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[[Category: Manchak, J.]]
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[[Category: Manchak J]]
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[[Category: Scott, P.G.]]
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[[Category: Scott PG]]
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[[Category: Wong, J.J.]]
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[[Category: Wong JJ]]
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[[Category: four helix bundle]]
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[[Category: tetramer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:00:49 2007''
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Current revision

Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM

PDB ID 2g7o

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