This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2hyp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:00, 21 February 2024) (edit) (undo)
 
(8 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2hyp.png|left|200px]]
 
-
<!--
+
==Crystal structure of Rv0805 D66A mutant==
-
The line below this paragraph, containing "STRUCTURE_2hyp", creates the "Structure Box" on the page.
+
<StructureSection load='2hyp' size='340' side='right'caption='[[2hyp]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2hyp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HYP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HYP FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-
{{STRUCTURE_2hyp| PDB=2hyp | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hyp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hyp OCA], [https://pdbe.org/2hyp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hyp RCSB], [https://www.ebi.ac.uk/pdbsum/2hyp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hyp ProSAT]</span></td></tr>
-
 
+
</table>
-
===Crystal structure of Rv0805 D66A mutant===
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/CNPD3_MYCTU CNPD3_MYCTU] Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP (PubMed:16313172, PubMed:18757371). Can use 2',3'-cAMP, 2',3'-cGMP, 3',5'-cAMP, 3',5'-cGMP and 3',5'-cUMP (PubMed:16313172, PubMed:18757371, PubMed:19801656). Hydrolysis of 2',3'-cAMP produces a mixture of 3'-AMP (major product) and 2'-AMP (minor product) (PubMed:18757371). In vitro, is 150-fold more active in hydrolyzing 2',3'-cAMP than 3',5'-cAMP (PubMed:18757371). Can also hydrolyze the model substrates p-nitrophenyl phosphate (pNPP), bis-(p-nitrophenyl phosphate) (bis(pNPP)) and p-nitrophenyl phenylphosphonate (pNPPP) (PubMed:16313172, PubMed:18757371, PubMed:19801656). Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes (PubMed:16313172). May play a role in pathogenicity, not only by hydrolyzing cAMP, but also by altering properties of the cell wall (PubMed:19801656).<ref>PMID:16313172</ref> <ref>PMID:18757371</ref> <ref>PMID:19801656</ref> Overexpression elicits a transcriptional response that is independent of the phosphodiesterase activity. It does not alter the levels of cAMP-CRP regulated genes, even though cAMP levels are reduced in cells.<ref>PMID:23835087</ref>
-
 
+
== Evolutionary Conservation ==
-
<!--
+
[[Image:Consurf_key_small.gif|200px|right]]
-
The line below this paragraph, {{ABSTRACT_PUBMED_17059828}}, adds the Publication Abstract to the page
+
Check<jmol>
-
(as it appears on PubMed at http://www.pubmed.gov), where 17059828 is the PubMed ID number.
+
<jmolCheckbox>
-
-->
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hy/2hyp_consurf.spt"</scriptWhenChecked>
-
{{ABSTRACT_PUBMED_17059828}}
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==About this Structure==
+
</jmolCheckbox>
-
2HYP is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HYP OCA].
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hyp ConSurf].
-
 
+
<div style="clear:both"></div>
-
==Reference==
+
== References ==
-
<ref group="xtra">PMID:17059828</ref><ref group="xtra">PMID:16313172</ref><references group="xtra"/>
+
<references/>
-
[[Category: 3',5'-cyclic-nucleotide phosphodiesterase]]
+
__TOC__
-
[[Category: Mycobacterium tuberculosis]]
+
</StructureSection>
-
[[Category: Capuder, M.]]
+
[[Category: Large Structures]]
-
[[Category: Draskovic, P.]]
+
[[Category: Mycobacterium tuberculosis H37Rv]]
-
[[Category: Lamba, D.]]
+
[[Category: Capuder M]]
-
[[Category: Podobnik, M.]]
+
[[Category: Draskovic P]]
-
[[Category: Shenoy, A R.]]
+
[[Category: Lamba D]]
-
[[Category: Visweswariah, S S.]]
+
[[Category: Podobnik M]]
-
[[Category: Bi-nuclear active site]]
+
[[Category: Shenoy AR]]
-
[[Category: Camp]]
+
[[Category: Visweswariah SS]]
-
[[Category: Metallophosphoesterase]]
+
-
[[Category: Phosphodiesterase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:04:55 2009''
+

Current revision

Crystal structure of Rv0805 D66A mutant

PDB ID 2hyp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hyp"
Personal tools