1efk

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{{Seed}}
 
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[[Image:1efk.png|left|200px]]
 
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==STRUCTURE OF HUMAN MALIC ENZYME IN COMPLEX WITH KETOMALONATE==
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The line below this paragraph, containing "STRUCTURE_1efk", creates the "Structure Box" on the page.
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<StructureSection load='1efk' size='340' side='right'caption='[[1efk]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1efk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EFK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAK:ALPHA-KETOMALONIC+ACID'>MAK</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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{{STRUCTURE_1efk| PDB=1efk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1efk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1efk OCA], [https://pdbe.org/1efk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1efk RCSB], [https://www.ebi.ac.uk/pdbsum/1efk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1efk ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ef/1efk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1efk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Malic enzymes are widely distributed in nature and have many biological functions. The crystal structure of human mitochondrial NAD(P)+-dependent malic enzyme in a quaternary complex with NAD+, Mn++ and oxalate has been determined at 2.2 A resolution. The structures of the quaternary complex with NAD+, Mg++, tartronate or ketomalonate have been determined at 2.6 A resolution. The structures show the enzyme in a closed form in these complexes and reveal the binding modes of the cation and the inhibitors. The divalent cation is coordinated in an octahedral fashion by six ligating oxygens, two from the substrate/inhibitor, three from Glu 255, Asp 256 and Asp 279 of the enzyme, and one from a water molecule. The structural information has significant implications for the catalytic mechanism of malic enzymes and identifies Tyr 112 and Lys 183 as possible catalytic residues. Changes in tetramer organization of the enzyme are also observed in these complexes, which might be relevant for its cooperative behavior and allosteric control.
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===STRUCTURE OF HUMAN MALIC ENZYME IN COMPLEX WITH KETOMALONATE===
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Structure of a closed form of human malic enzyme and implications for catalytic mechanism.,Yang Z, Floyd DL, Loeber G, Tong L Nat Struct Biol. 2000 Mar;7(3):251-7. PMID:10700286<ref>PMID:10700286</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_10700286}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1efk" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 10700286 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_10700286}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1EFK is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFK OCA].
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==Reference==
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<ref group="xtra">PMID:10700286</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Floyd, D L.]]
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[[Category: Large Structures]]
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[[Category: Loeber, G.]]
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[[Category: Floyd DL]]
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[[Category: Tong, L.]]
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[[Category: Loeber G]]
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[[Category: Yang, Z.]]
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[[Category: Tong L]]
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[[Category: Closed form]]
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[[Category: Yang Z]]
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[[Category: Malic enzyme]]
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[[Category: Quaternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:14:37 2009''
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Current revision

STRUCTURE OF HUMAN MALIC ENZYME IN COMPLEX WITH KETOMALONATE

PDB ID 1efk

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