2o97

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{{Seed}}
 
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[[Image:2o97.png|left|200px]]
 
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==Crystal Structure of E. coli HU heterodimer==
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The line below this paragraph, containing "STRUCTURE_2o97", creates the "Structure Box" on the page.
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<StructureSection load='2o97' size='340' side='right'caption='[[2o97]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2o97]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O97 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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{{STRUCTURE_2o97| PDB=2o97 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o97 OCA], [https://pdbe.org/2o97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o97 RCSB], [https://www.ebi.ac.uk/pdbsum/2o97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o97 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DBHA_ECOLI DBHA_ECOLI] Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o9/2o97_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o97 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We determined the crystal structure of the Escherichia coli nucleoid-associated HUalphabeta protein by x-ray diffraction and observed that the heterodimers form multimers with octameric units in three potential arrangements, which may serve specialized roles in different DNA transaction reactions. It is of special importance that one of the structures forms spiral filaments with left-handed rotations. A negatively superhelical DNA can be modeled to wrap around this left-handed HUalphabeta multimer. Whereas the wild-type HU generated negative DNA supercoiling in vitro, an engineered heterodimer with an altered amino acid residue critical for the formation of the left-handed spiral protein in the crystal was defective in the process, thus providing the structural explanation for the classical property of HU to restrain negative supercoils in DNA.
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===Crystal Structure of E. coli HU heterodimer===
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Spiral structure of Escherichia coli HUalphabeta provides foundation for DNA supercoiling.,Guo F, Adhya S Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4309-14. Epub 2007 Mar 5. PMID:17360520<ref>PMID:17360520</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17360520}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2o97" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17360520 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17360520}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2O97 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O97 OCA].
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==Reference==
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<ref group="xtra">PMID:17360520</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Adhya, S.]]
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[[Category: Large Structures]]
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[[Category: Guo, F.]]
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[[Category: Adhya S]]
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[[Category: Dna structure]]
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[[Category: Guo F]]
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[[Category: Dna supercoiling]]
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[[Category: Dna-binding]]
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[[Category: E. coli]]
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[[Category: Heterodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:19:31 2009''
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Current revision

Crystal Structure of E. coli HU heterodimer

PDB ID 2o97

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OCA

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