2ga9

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(New page: 200px<br /><applet load="2ga9" size="450" color="white" frame="true" align="right" spinBox="true" caption="2ga9, resolution 2.300&Aring;" /> '''Crystal Structure o...)
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[[Image:2ga9.gif|left|200px]]<br /><applet load="2ga9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ga9, resolution 2.300&Aring;" />
 
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'''Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S'''<br />
 
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==Overview==
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==Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S==
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Polyadenylation of mRNAs in poxviruses, crucial for virion maturation, is, carried out by a poly(A) polymerase heterodimer composed of a catalytic, component, VP55, and a processivity factor, VP39. The ATP-gamma-S bound, and unbound crystal structures of the vaccinia polymerase reveal an, unusual architecture for VP55 that comprises of N-terminal, central or, catalytic, and C-terminal domains with different topologies and that, differs from many polymerases, including the eukaryotic poly(A), polymerases. Residues in the active site of VP55, located between the, catalytic and C-terminal domains, make specific interactions with the, adenine of the ATP analog, establishing the molecular basis of ATP, recognition. VP55's concave surface docks the globular VP39. A model for, RNA primer binding that involves all three VP55 domains and VP39 is, proposed. The model supports biochemical evidence that VP39 functions as a, processivity factor by partially enclosing the RNA primer at the, heterodimer interface.
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<StructureSection load='2ga9' size='340' side='right'caption='[[2ga9]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ga9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GA9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GA9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ga9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ga9 OCA], [https://pdbe.org/2ga9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ga9 RCSB], [https://www.ebi.ac.uk/pdbsum/2ga9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ga9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MCE_VACCW MCE_VACCW] Displays methyltransferase, positive regulation of the poly(A) polymerase and transcription elongation activities. Involved in the modification of both mRNA ends and in intermediate and late gene positive transcription elongation. At the mRNAs 5' end, methylates the ribose 2' OH group of the first transcribed nucleotide, thereby producing a 2'-O-methylpurine cap. At the 3' end, functions as a processivity factor which stimulates the activity of the viral poly(A) polymerase VP55 that creates mRNA's poly(A) tail. In the presence of VP39, VP55 does not dissociate from the RNA allowing tail elongation to around 250 adenylates.<ref>PMID:1313572</ref> <ref>PMID:1670500</ref> <ref>PMID:12359447</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/2ga9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ga9 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GA9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Vaccinia_virus Vaccinia virus] with CA and SAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/mRNA_(nucleoside-2'-O-)-methyltransferase mRNA (nucleoside-2'-O-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.57 2.1.1.57] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GA9 OCA].
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*[[Poly(A) polymerase 3D structures|Poly(A) polymerase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity., Moure CM, Bowman BR, Gershon PD, Quiocho FA, Mol Cell. 2006 May 5;22(3):339-49. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16678106 16678106]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vaccinia virus]]
[[Category: Vaccinia virus]]
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[[Category: mRNA (nucleoside-2'-O-)-methyltransferase]]
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[[Category: Bowman BR]]
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[[Category: Bowman, B.R.]]
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[[Category: Gershon PD]]
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[[Category: Gershon, P.D.]]
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[[Category: Moure CM]]
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[[Category: Moure, C.M.]]
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[[Category: Quiocho FA]]
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[[Category: Quiocho, F.A.]]
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[[Category: CA]]
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[[Category: SAP]]
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[[Category: heterodimer]]
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[[Category: nucleotidyltransferase]]
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[[Category: polyadenylate polymerase]]
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[[Category: poxvirus]]
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[[Category: processivity]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:04:33 2007''
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Current revision

Crystal Structure of the Heterodimeric Vaccinia Virus Polyadenylate Polymerase with Bound ATP-gamma-S

PDB ID 2ga9

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