3e7x

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{{Seed}}
 
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[[Image:3e7x.png|left|200px]]
 
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==Crystal structure of DLTA: implications for the reaction mechanism of non-ribosomal peptide synthetase (NRPS) adenylation domains==
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The line below this paragraph, containing "STRUCTURE_3e7x", creates the "Structure Box" on the page.
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<StructureSection load='3e7x' size='340' side='right'caption='[[3e7x]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3e7x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E7X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E7X FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
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{{STRUCTURE_3e7x| PDB=3e7x | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e7x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e7x OCA], [https://pdbe.org/3e7x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e7x RCSB], [https://www.ebi.ac.uk/pdbsum/3e7x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e7x ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DLTA_BACSU DLTA_BACSU] Involved in the biosynthesis of D-alanyl-lipoteichoic acid (LTA). Catalyzes an ATP-dependent two-step reaction where it forms a high energy D-alanyl AMP intermediate and transfers the alanyl residues from AMP to Dcp.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e7/3e7x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3e7x ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DltA, the D-alanine:D-alanyl carrier protein ligase responsible for the initial step of lipoteichoic acid D-alanylation in Gram-positive bacteria, belongs to the adenylation domain superfamily, which also includes acetyl-CoA synthetase and the adenylation domains of non-ribosomal synthetases. The two-step reaction catalyzed by these enzymes (substrate adenylation followed by transfer to the reactive thiol group of CoA or the phosphopantheinyl prosthetic group of peptidyl carrier proteins) has been suggested to proceed via large scale rearrangements of structural domains within the enzyme. The structures of DltA reported here reveal the determinants for D-Ala substrate specificity and confirm that the peptidyl carrier protein-activating domains are able to adopt multiple conformational states, in this case corresponding to the thiolation reaction. Comparisons of available structures allow us to propose a mechanism whereby small perturbations of finely balanced metastable structural states would be able to direct an ordered formation of non-ribosomal synthetase products.
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===Crystal structure of DLTA: implications for the reaction mechanism of non-ribosomal peptide synthetase (NRPS) adenylation domains===
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Crystal structure of DltA. Implications for the reaction mechanism of non-ribosomal peptide synthetase adenylation domains.,Yonus H, Neumann P, Zimmermann S, May JJ, Marahiel MA, Stubbs MT J Biol Chem. 2008 Nov 21;283(47):32484-91. Epub 2008 Sep 10. PMID:18784082<ref>PMID:18784082</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_18784082}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3e7x" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18784082 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18784082}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3E7X is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E7X OCA].
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==Reference==
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<ref group="xtra">PMID:18784082</ref><references group="xtra"/>
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Marahiel, M A.]]
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[[Category: Large Structures]]
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[[Category: May, J J.]]
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[[Category: Marahiel MA]]
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[[Category: Neumann, P.]]
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[[Category: May JJ]]
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[[Category: Stubbs, M T.]]
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[[Category: Neumann P]]
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[[Category: Yonus, H.]]
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[[Category: Stubbs MT]]
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[[Category: Zimmermann, S.]]
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[[Category: Yonus H]]
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[[Category: Adenylation domain]]
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[[Category: Zimmermann S]]
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[[Category: Amp]]
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[[Category: Cytoplasm]]
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[[Category: D-alanine-dalanyl]]
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[[Category: D-alanylation]]
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[[Category: Dlta]]
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[[Category: Ligase]]
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[[Category: Non-ribosomal peptide synthetase]]
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[[Category: Nrp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:32:26 2009''
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Current revision

Crystal structure of DLTA: implications for the reaction mechanism of non-ribosomal peptide synthetase (NRPS) adenylation domains

PDB ID 3e7x

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