3bx1

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{{Seed}}
 
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[[Image:3bx1.png|left|200px]]
 
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==Complex between the Barley alpha-Amylase/Subtilisin Inhibitor and the subtilisin Savinase==
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The line below this paragraph, containing "STRUCTURE_3bx1", creates the "Structure Box" on the page.
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<StructureSection load='3bx1' size='340' side='right'caption='[[3bx1]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3bx1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare] and [https://en.wikipedia.org/wiki/Lederbergia_lenta Lederbergia lenta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BX1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BX1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3bx1| PDB=3bx1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bx1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bx1 OCA], [https://pdbe.org/3bx1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bx1 RCSB], [https://www.ebi.ac.uk/pdbsum/3bx1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bx1 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SUBS_LEDLE SUBS_LEDLE] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bx/3bx1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bx1 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Subtilisins represent a large class of microbial serine proteases. To date, there are three-dimensional structures of proteinaceous inhibitors from three families in complex with subtilisins in the Protein Data Bank. All interact with subtilisin via an exposed loop covering six interacting residues. Here we present the crystal structure of the complex between the Bacillus lentus subtilisin Savinase and the barley alpha-amylase/subtilisin inhibitor (BASI). This is the first reported structure of a cereal Kunitz-P family inhibitor in complex with a subtilisin. Structural analysis revealed that BASI inhibits Savinase in a novel way, as the interacting loop is shorter than loops previously reported. Mutational analysis showed that Thr88 is crucial for the inhibition, as it stabilises the interacting loop through intramolecular interactions with the BASI backbone.
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===Complex between the Barley alpha-Amylase/Subtilisin Inhibitor and the subtilisin Savinase===
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Structural and mutational analyses of the interaction between the barley alpha-amylase/subtilisin inhibitor and the subtilisin savinase reveal a novel mode of inhibition.,Micheelsen PO, Vevodova J, De Maria L, Ostergaard PR, Friis EP, Wilson K, Skjot M J Mol Biol. 2008 Jul 18;380(4):681-90. Epub 2008 May 22. PMID:18556023<ref>PMID:18556023</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3bx1" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18556023}}, adds the Publication Abstract to the page
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*[[Subtilisin 3D structures|Subtilisin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18556023 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18556023}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3BX1 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_lentus Bacillus lentus] and [http://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BX1 OCA].
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==Reference==
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<ref group="xtra">PMID:18556023</ref><references group="xtra"/>
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[[Category: Bacillus lentus]]
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[[Category: Hordeum vulgare]]
[[Category: Hordeum vulgare]]
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[[Category: Subtilisin]]
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[[Category: Large Structures]]
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[[Category: Micheelsen, P O.]]
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[[Category: Lederbergia lenta]]
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[[Category: Skjot, M.]]
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[[Category: Micheelsen PO]]
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[[Category: Vevodova, J.]]
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[[Category: Skjot M]]
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[[Category: Wilson, K.]]
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[[Category: Vevodova J]]
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[[Category: Alpha-amylase inhibitor]]
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[[Category: Wilson K]]
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[[Category: Barley alpha-amylase/subtilisin inhibitor]]
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[[Category: Calcium]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase/hydrolase inhibitor complex]]
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[[Category: Metal-binding]]
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[[Category: Protease]]
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[[Category: Protease inhibitor]]
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[[Category: Savinase]]
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[[Category: Secreted]]
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[[Category: Serine protease]]
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[[Category: Serine protease inhibitor]]
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[[Category: Sporulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:35:42 2009''
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Current revision

Complex between the Barley alpha-Amylase/Subtilisin Inhibitor and the subtilisin Savinase

PDB ID 3bx1

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