2gbp

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(New page: 200px<br /><applet load="2gbp" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gbp, resolution 1.9&Aring;" /> '''SUGAR AND SIGNAL-TRAN...)
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[[Image:2gbp.gif|left|200px]]<br /><applet load="2gbp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gbp, resolution 1.9&Aring;" />
 
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'''SUGAR AND SIGNAL-TRANSDUCER BINDING SITES OF THE ESCHERICHIA COLI GALACTOSE CHEMORECEPTOR PROTEIN'''<br />
 
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==Overview==
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==SUGAR AND SIGNAL-TRANSDUCER BINDING SITES OF THE ESCHERICHIA COLI GALACTOSE CHEMORECEPTOR PROTEIN==
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D-galactose-binding (or chemoreceptor) protein of Escherichia coli serves, as an initial component for both chemotaxis towards galactose and glucose, and high-affinity active transport of the two sugars. Well-refined x-ray, structures of the liganded forms of the wild-type and a mutant protein, isolated from a strain defective in chemotaxis but fully competent in, transport have provided a molecular view of the sugar-binding site and of, a site for interacting with the Trg transmembrane signal transducer. The, geometry of the sugar-binding site, located in the cleft between the two, lobes of the bilobate protein, is novel in that it is designed for tight, binding and sequestering of either the alpha or beta anomer of the, D-stereoisomer of the 4-epimers galactose and glucose. Binding specificity, and affinity are conferred primarily by polar planar side-chain residues, that form intricate networks of cooperative and bidentate hydrogen bonds, with the sugar substrates, and secondarily by aromatic residues that, sandwich the pyranose ring. Each of the pairs of anomeric hydroxyls and, epimeric hydroxyls is recognized by a distinct Asp residue. The site for, interaction with the transducer is about 18 A from the sugar-binding site., Mutation of Gly74 to Asp at this site, concomitant with considerable, changes in the local ordered water structures, contributes to the lack of, productive interaction with the transmembrane signal transducer.
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<StructureSection load='2gbp' size='340' side='right'caption='[[2gbp]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2gbp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GBP FirstGlance]. <br>
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2GBP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with GLC and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GBP OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gbp OCA], [https://pdbe.org/2gbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gbp RCSB], [https://www.ebi.ac.uk/pdbsum/2gbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gbp ProSAT]</span></td></tr>
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Sugar and signal-transducer binding sites of the Escherichia coli galactose chemoreceptor protein., Vyas NK, Vyas MN, Quiocho FA, Science. 1988 Dec 2;242(4883):1290-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=3057628 3057628]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MGLB_ECOLI MGLB_ECOLI] Part of the ABC transporter complex MglABC involved in galactose/methyl galactoside import (Probable). In addition, binds D-galactose and D-glucose and plays a role in the chemotaxis towards these two sugars by interacting with the Trg chemoreceptor (PubMed:3057628, PubMed:4927373). Chemotaxis requires MglB, but not MglA or MglC (PubMed:6294056).<ref>PMID:3057628</ref> <ref>PMID:4927373</ref> <ref>PMID:6294056</ref> <ref>PMID:1719366</ref> <ref>PMID:6294056</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gb/2gbp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gbp ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Quiocho, F.A.]]
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[[Category: Quiocho FA]]
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[[Category: Vyas, M.N.]]
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[[Category: Vyas MN]]
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[[Category: Vyas, N.K.]]
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[[Category: Vyas NK]]
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[[Category: CA]]
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[[Category: GLC]]
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[[Category: periplasmic binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:06:07 2007''
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SUGAR AND SIGNAL-TRANSDUCER BINDING SITES OF THE ESCHERICHIA COLI GALACTOSE CHEMORECEPTOR PROTEIN

PDB ID 2gbp

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