1t3k

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{{Seed}}
 
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[[Image:1t3k.png|left|200px]]
 
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==NMR structure of a CDC25-like dual-specificity tyrosine phosphatase of Arabidopsis thaliana==
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The line below this paragraph, containing "STRUCTURE_1t3k", creates the "Structure Box" on the page.
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<StructureSection load='1t3k' size='340' side='right'caption='[[1t3k]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1t3k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T3K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1t3k| PDB=1t3k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t3k OCA], [https://pdbe.org/1t3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t3k RCSB], [https://www.ebi.ac.uk/pdbsum/1t3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t3k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CDC25_ARATH CDC25_ARATH] Tyrosine protein phosphatase that dephosphorylates CDK complex and activate its kinase activity in vitro.<ref>PMID:16567632</ref> <ref>PMID:15329414</ref> Arsenate reductase that plays a major role in the reduction of arsenate to arsenite and arsenic retention in roots.<ref>PMID:16567632</ref> <ref>PMID:15329414</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t3/1t3k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t3k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The dual-specificity CDC25 phosphatases are critical positive regulators of cyclin-dependent kinases (CDKs). Even though an antagonistic Arabidopsis thaliana WEE1 kinase has been cloned and tyrosine phosphorylation of its CDKs has been demonstrated, no valid candidate for a CDC25 protein has been reported in higher plants. We identify a CDC25-related protein (Arath;CDC25) of A. thaliana, constituted by a sole catalytic domain. The protein has a tyrosine-phosphatase activity and stimulates the kinase activity of Arabidopsis CDKs. Its tertiary structure was obtained by NMR spectroscopy and confirms that Arath;CDC25 belongs structurally to the classical CDC25 superfamily with a central five-stranded beta-sheet surrounded by helices. A particular feature of the protein, however, is the presence of an additional zinc-binding loop in the C-terminal part. NMR mapping studies revealed the interaction with phosphorylated peptidic models derived from the conserved CDK loop containing the phosphothreonine-14 and phosphotyrosine-15. We conclude that despite sequence divergence, Arath;CDC25 is structurally and functionally an isoform of the CDC25 superfamily, which is conserved in yeast and in plants, including Arabidopsis and rice.
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===NMR structure of a CDC25-like dual-specificity tyrosine phosphatase of Arabidopsis thaliana===
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A small CDC25 dual-specificity tyrosine-phosphatase isoform in Arabidopsis thaliana.,Landrieu I, da Costa M, De Veylder L, Dewitte F, Vandepoele K, Hassan S, Wieruszeski JM, Corellou F, Faure JD, Van Montagu M, Inze D, Lippens G Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13380-5. Epub 2004 Aug 25. PMID:15329414<ref>PMID:15329414</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1t3k" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15329414}}, adds the Publication Abstract to the page
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*[[Dual specificity phosphatase 3D structures|Dual specificity phosphatase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15329414 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15329414}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1T3K is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3K OCA].
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==Reference==
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<ref group="xtra">PMID:15329414</ref><references group="xtra"/>
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[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Costa, M da.]]
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[[Category: De Veylder L]]
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[[Category: Dewitte, F.]]
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[[Category: Dewitte F]]
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[[Category: Faure, J D.]]
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[[Category: Faure JD]]
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[[Category: Hassan, S.]]
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[[Category: Hassan S]]
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[[Category: Inze, D.]]
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[[Category: Inze D]]
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[[Category: Landrieu, I.]]
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[[Category: Landrieu I]]
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[[Category: Lippens, G.]]
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[[Category: Lippens G]]
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[[Category: Vandepoele, K.]]
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[[Category: Vandepoele K]]
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[[Category: Veylder, L De.]]
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[[Category: Wieruszeski JM]]
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[[Category: Wieruszeski, J M.]]
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[[Category: Da Costa M]]
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[[Category: Cdc25]]
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[[Category: Cell cycle]]
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[[Category: Phosphorylation]]
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[[Category: Plant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:38:31 2009''
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Current revision

NMR structure of a CDC25-like dual-specificity tyrosine phosphatase of Arabidopsis thaliana

PDB ID 1t3k

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