2gdw

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(New page: 200px<br /><applet load="2gdw" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gdw" /> '''Solution structure of the B. brevis TycC3-PC...)
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[[Image:2gdw.gif|left|200px]]<br /><applet load="2gdw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gdw" />
 
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'''Solution structure of the B. brevis TycC3-PCP in A/H-state'''<br />
 
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==Overview==
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==Solution structure of the B. brevis TycC3-PCP in A/H-state==
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Protein dynamics plays an important role in protein function. Many, functionally important motions occur on the microsecond and low, millisecond time scale and can be characterized by nuclear magnetic, resonance relaxation experiments. We describe the different states of a, peptidyl carrier protein (PCP) that play a crucial role in its function as, a peptide shuttle in the nonribosomal peptide synthetases of the, tyrocidine A system. Both apo-PCP (without the bound 4'-phosphopantetheine, cofactor) and holo-PCP exist in two different stable conformations. We, show that one of the apo conformations and one of the holo conformations, are identical, whereas the two remaining conformations are only detectable, by nuclear magnetic resonance spectroscopy in either the apo or holo form., We further demonstrate that this conformational diversity is an essential, prerequisite for the directed movement of the 4'-PP cofactor and its, interaction with externally acting proteins such as thioesterases and, 4'-PP transferase.
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<StructureSection load='2gdw' size='340' side='right'caption='[[2gdw]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gdw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevibacillus_parabrevis Brevibacillus parabrevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GDW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gdw OCA], [https://pdbe.org/2gdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gdw RCSB], [https://www.ebi.ac.uk/pdbsum/2gdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gdw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TYCC_BREPA TYCC_BREPA] Incorporates six amino acids (for tyrocidine A, Asn, Gln, Tyr, Val, Orn, and Leu) in their L-configuration into the peptide product.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gd/2gdw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gdw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein dynamics plays an important role in protein function. Many functionally important motions occur on the microsecond and low millisecond time scale and can be characterized by nuclear magnetic resonance relaxation experiments. We describe the different states of a peptidyl carrier protein (PCP) that play a crucial role in its function as a peptide shuttle in the nonribosomal peptide synthetases of the tyrocidine A system. Both apo-PCP (without the bound 4'-phosphopantetheine cofactor) and holo-PCP exist in two different stable conformations. We show that one of the apo conformations and one of the holo conformations are identical, whereas the two remaining conformations are only detectable by nuclear magnetic resonance spectroscopy in either the apo or holo form. We further demonstrate that this conformational diversity is an essential prerequisite for the directed movement of the 4'-PP cofactor and its interaction with externally acting proteins such as thioesterases and 4'-PP transferase.
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==About this Structure==
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Conformational switches modulate protein interactions in peptide antibiotic synthetases.,Koglin A, Mofid MR, Lohr F, Schafer B, Rogov VV, Blum MM, Mittag T, Marahiel MA, Bernhard F, Dotsch V Science. 2006 Apr 14;312(5771):273-6. PMID:16614225<ref>PMID:16614225</ref>
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2GDW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Brevibacillus_parabrevis Brevibacillus parabrevis]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GDW OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Conformational switches modulate protein interactions in peptide antibiotic synthetases., Koglin A, Mofid MR, Lohr F, Schafer B, Rogov VV, Blum MM, Mittag T, Marahiel MA, Bernhard F, Dotsch V, Science. 2006 Apr 14;312(5771):273-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16614225 16614225]
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</div>
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<div class="pdbe-citations 2gdw" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Brevibacillus parabrevis]]
[[Category: Brevibacillus parabrevis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bernhard, F.]]
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[[Category: Bernhard F]]
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[[Category: Doetsch, V.]]
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[[Category: Doetsch V]]
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[[Category: Koglin, A.]]
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[[Category: Koglin A]]
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[[Category: Loehr, F.]]
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[[Category: Loehr F]]
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[[Category: Marahiel, M.A.]]
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[[Category: Marahiel MA]]
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[[Category: Rogov, V.V.]]
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[[Category: Rogov VV]]
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[[Category: three-helix bundle]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:08:43 2007''
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Current revision

Solution structure of the B. brevis TycC3-PCP in A/H-state

PDB ID 2gdw

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