1onv

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{{Seed}}
 
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[[Image:1onv.png|left|200px]]
 
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==NMR Structure of a Complex Containing the TFIIF Subunit RAP74 and the RNAP II CTD Phosphatase FCP1==
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The line below this paragraph, containing "STRUCTURE_1onv", creates the "Structure Box" on the page.
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<StructureSection load='1onv' size='340' side='right'caption='[[1onv]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1onv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ONV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ONV FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1onv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1onv OCA], [https://pdbe.org/1onv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1onv RCSB], [https://www.ebi.ac.uk/pdbsum/1onv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1onv ProSAT]</span></td></tr>
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{{STRUCTURE_1onv| PDB=1onv | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/T2FA_HUMAN T2FA_HUMAN] TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.<ref>PMID:10428810</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/1onv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1onv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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FCP1 [transcription factor IIF (TFIIF)-associated carboxyl-terminal domain (CTD) phosphatase] is the only identified phosphatase specific for the phosphorylated CTD of RNA polymerase II (RNAP II). The phosphatase activity of FCP1 is enhanced in the presence of the large subunit of TFIIF (RAP74 in humans). It has been demonstrated that the CTD of RAP74 (cterRAP74; residues 436-517) directly interacts with the highly acidic CTD of FCP1 (cterFCP; residues 879-961 in human). In this manuscript, we have determined a high-resolution solution structure of a cterRAP74cterFCP complex by NMR spectroscopy. Interestingly, the cterFCP protein is completely disordered in the unbound state, but forms an alpha-helix (H1'; E945-M961) in the complex. The cterRAP74cterFCP binding interface relies extensively on van der Waals contacts between hydrophobic residues from the H2 and H3 helices of cterRAP74 and hydrophobic residues from the H1' helix of cterFCP. The binding interface also contains two critical electrostatic interactions involving aspartic acid residues from H1' of cterFCP and lysine residues from both H2 and H3 of cterRAP74. There are also three additional polar interactions involving highly conserved acidic residues from the H1' helix. The cterRAP74cterFCP complex is the first high-resolution structure between an acidic residue-rich domain from a holoenzyme-associated regulatory protein and a general transcription factor. The structure defines a clear role for both hydrophobic and acidic residues in proteinprotein complexes involving acidic residue-rich domains in transcription regulatory proteins.
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===NMR Structure of a Complex Containing the TFIIF Subunit RAP74 and the RNAP II CTD Phosphatase FCP1===
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NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1.,Nguyen BD, Abbott KL, Potempa K, Kobor MS, Archambault J, Greenblatt J, Legault P, Omichinski JG Proc Natl Acad Sci U S A. 2003 May 13;100(10):5688-93. Epub 2003 May 5. PMID:12732728<ref>PMID:12732728</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1onv" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12732728}}, adds the Publication Abstract to the page
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*[[Transcription initiation factors 3D structures|Transcription initiation factors 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12732728 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12732728}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1ONV is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ONV OCA].
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==Reference==
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<ref group="xtra">PMID:12732728</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Abbott, K L.]]
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[[Category: Large Structures]]
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[[Category: Archambault, J.]]
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[[Category: Abbott KL]]
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[[Category: Greenblatt, J.]]
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[[Category: Archambault J]]
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[[Category: Kobor, M S.]]
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[[Category: Greenblatt J]]
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[[Category: Legault, P.]]
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[[Category: Kobor MS]]
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[[Category: Nguyen, B D.]]
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[[Category: Legault P]]
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[[Category: Omichinski, J G.]]
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[[Category: Nguyen BD]]
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[[Category: Potempa, K.]]
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[[Category: Omichinski JG]]
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[[Category: Fcp1]]
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[[Category: Potempa K]]
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[[Category: Human general transcription factor tfiif]]
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[[Category: Rap74]]
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[[Category: Rna polymerase ii ctd phosphatase]]
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[[Category: Tfiif-associating ctd phosphatase]]
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[[Category: Transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:01:23 2009''
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Current revision

NMR Structure of a Complex Containing the TFIIF Subunit RAP74 and the RNAP II CTD Phosphatase FCP1

PDB ID 1onv

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