1s2h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:07, 22 May 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1s2h.png|left|200px]]
 
-
<!--
+
==The Mad2 spindle checkpoint protein possesses two distinct natively folded states==
-
The line below this paragraph, containing "STRUCTURE_1s2h", creates the "Structure Box" on the page.
+
<StructureSection load='1s2h' size='340' side='right'caption='[[1s2h]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1s2h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S2H FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s2h OCA], [https://pdbe.org/1s2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s2h RCSB], [https://www.ebi.ac.uk/pdbsum/1s2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s2h ProSAT]</span></td></tr>
-
{{STRUCTURE_1s2h| PDB=1s2h | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MD2L1_HUMAN MD2L1_HUMAN] Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.<ref>PMID:10700282</ref> <ref>PMID:11804586</ref> <ref>PMID:15024386</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s2/1s2h_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s2h ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The spindle checkpoint delays chromosome segregation in response to misaligned sister chromatids during mitosis, thus ensuring the fidelity of chromosome inheritance. Through binding to Cdc20, the Mad2 spindle checkpoint protein inhibits the target of this checkpoint, the ubiquitin protein ligase APC/C(Cdc20). We now show that without cofactor binding or covalent modification Mad2 adopts two distinct folded conformations at equilibrium (termed N1-Mad2 and N2-Mad2). The structure of N2-Mad2 has been determined by NMR spectroscopy. N2-Mad2 is much more potent in APC/C inhibition. Overexpression of a Mad2 mutant that specifically sequesters N2-Mad2 partially blocks checkpoint signaling in living cells. The two Mad2 conformers interconvert slowly in vitro, but interconversion is accelerated by a fragment of Mad1, an upstream regulator of Mad2. Our results suggest that the unusual two-state behavior of Mad2 is critical for spindle checkpoint signaling.
-
===The Mad2 spindle checkpoint protein possesses two distinct natively folded states===
+
The Mad2 spindle checkpoint protein has two distinct natively folded states.,Luo X, Tang Z, Xia G, Wassmann K, Matsumoto T, Rizo J, Yu H Nat Struct Mol Biol. 2004 Apr;11(4):338-45. Epub 2004 Mar 14. PMID:15024386<ref>PMID:15024386</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_15024386}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 1s2h" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 15024386 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_15024386}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1S2H is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S2H OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:15024386</ref><references group="xtra"/>
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Luo, X.]]
+
[[Category: Large Structures]]
-
[[Category: Matsumoto, T.]]
+
[[Category: Luo X]]
-
[[Category: Rizo, J.]]
+
[[Category: Matsumoto T]]
-
[[Category: Tang, Z.]]
+
[[Category: Rizo J]]
-
[[Category: Wassmann, K.]]
+
[[Category: Tang Z]]
-
[[Category: Xia, G.]]
+
[[Category: Wassmann K]]
-
[[Category: Yu, H.]]
+
[[Category: Xia G]]
-
[[Category: Mad2]]
+
[[Category: Yu H]]
-
[[Category: Spindle checkpoint protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 01:47:44 2009''
+

Current revision

The Mad2 spindle checkpoint protein possesses two distinct natively folded states

PDB ID 1s2h

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1s2h"
Personal tools