2gh7

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(New page: 200px<br /><applet load="2gh7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gh7, resolution 1.00&Aring;" /> '''Epi-biotin complex w...)
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[[Image:2gh7.gif|left|200px]]<br /><applet load="2gh7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gh7, resolution 1.00&Aring;" />
 
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'''Epi-biotin complex with core streptavidin'''<br />
 
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==Overview==
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==Epi-biotin complex with core streptavidin==
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(+)-Epi-biotin differs from (+)-biotin in the configuration of the chiral, center at atom C2. This could lead to a difference in the mode of binding, of (+)-epi-biotin to streptavidin, a natural protein receptor for, (+)-biotin. Diffraction data were collected to a maximum of 0.85 Angstrom, resolution for structural analysis of the complex of streptavidin with a, sample of (+)-epi-biotin and refinement was carried out at both 1.0 and, 0.85 Angstrom resolution. The structure determination shows a, superposition of two ligands in the binding site, (+)-biotin and, (+)-epi-biotin. The molecules overlap in the model for the complex except, for the position of S1 in the tetrahydrothiophene ring. Differences in the, conformation of the ring permits binding of each molecule to streptavidin, with little observable difference in the protein structures at this high, resolution.
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<StructureSection load='2gh7' size='340' side='right'caption='[[2gh7]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gh7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GH7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=BTQ:EPI-BIOTIN'>BTQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gh7 OCA], [https://pdbe.org/2gh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2gh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gh7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2gh7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gh7 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GH7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] with BTN, BTQ and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GH7 OCA].
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*[[Avidin 3D structures|Avidin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The high-resolution structure of (+)-epi-biotin bound to streptavidin., Le Trong I, Aubert DG, Thomas NR, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):576-81. Epub 2006, May 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16699183 16699183]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
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[[Category: Aubert, D.G.L.]]
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[[Category: Aubert DGL]]
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[[Category: Stenkamp, R.E.]]
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[[Category: Le Trong I]]
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[[Category: Thomas, N.R.]]
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[[Category: Stenkamp RE]]
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[[Category: Trong, I.Le.]]
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[[Category: Thomas NR]]
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[[Category: BTN]]
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[[Category: BTQ]]
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[[Category: GOL]]
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[[Category: biotin]]
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[[Category: protein/ligand interactions]]
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[[Category: streptavidin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:12:17 2007''
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Current revision

Epi-biotin complex with core streptavidin

PDB ID 2gh7

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