2gho

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(New page: 200px<br /><applet load="2gho" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gho, resolution 5.000&Aring;" /> '''Recombinant Thermus...)
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[[Image:2gho.gif|left|200px]]<br /><applet load="2gho" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gho, resolution 5.000&Aring;" />
 
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'''Recombinant Thermus aquaticus RNA polymerase for Structural Studies'''<br />
 
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==Overview==
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==Recombinant Thermus aquaticus RNA polymerase for Structural Studies==
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Advances in the structural biology of bacterial transcription have come, from studies of RNA polymerases (RNAPs) from the thermophilic eubacteria, Thermus aquaticus (Taq) and Thermus thermophilus (Tth). These structural, studies have been limited by the fact that only endogenous Taq or Tth, RNAP, laboriously purified from large quantities of Taq or Tth cell paste, and offering few options for genetic modification, is suitable for, structural studies. Recombinant systems for the preparation of Taq RNAP by, co-overexpression and assembly in the heterologous host, Escherichia coli, have been described, but these did not yield enzyme suitable for, crystallographic studies. Here we describe recombinant systems for the, preparation of Taq RNAP harboring full or partial deletions of the Taq, beta' non-conserved domain (NCD), yielding enzyme suitable for, crystallographic studies. This opens the way for structural studies of, genetically manipulated enzymes, allowing the preparation of more, crystallizable enzymes and facilitating detailed structure/function, analysis. Characterization of the Taqbeta'NCD deletion mutants generated, in this study showed that the beta'NCD is important for the efficient, binding of the sigma subunit, confirming previous hypotheses. Finally, preliminary structural analysis (at 4.1Angstroms resolution) of one of the, recombinant mutants revealed a previously unobserved conformation of the, beta-flap, further defining the range of conformations accessible to this, flexible structural element.
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<StructureSection load='2gho' size='340' side='right'caption='[[2gho]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gho]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GHO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gho FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gho OCA], [https://pdbe.org/2gho PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gho RCSB], [https://www.ebi.ac.uk/pdbsum/2gho PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gho ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_THEAQ RPOA_THEAQ]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2gho_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gho ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GHO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Active as [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GHO OCA].
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Recombinant Thermus aquaticus RNA polymerase for structural studies., Kuznedelov K, Lamour V, Patikoglou G, Chlenov M, Darst SA, Severinov K, J Mol Biol. 2006 May 26;359(1):110-21. Epub 2006 Mar 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16618493 16618493]
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[[Category: Large Structures]]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Protein complex]]
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[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
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[[Category: Darst, S.A.]]
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[[Category: Darst SA]]
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[[Category: Lamour, V.]]
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[[Category: Lamour V]]
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[[Category: coexpression]]
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[[Category: recombinant multiprotein complex]]
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[[Category: thermus aquaticus rna polymerase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:12:35 2007''
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Current revision

Recombinant Thermus aquaticus RNA polymerase for Structural Studies

PDB ID 2gho

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