2gic

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(New page: 200px<br /><applet load="2gic" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gic, resolution 2.920&Aring;" /> '''Crystal Structure o...)
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[[Image:2gic.gif|left|200px]]<br /><applet load="2gic" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2gic, resolution 2.920&Aring;" />
 
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'''Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex'''<br />
 
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==Overview==
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==Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex==
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Vesicular stomatitis virus is a negative-stranded RNA virus. Its, nucleoprotein (N) binds the viral genomic RNA and is involved in multiple, functions including transcription, replication, and assembly. We have, determined a 2.9 angstrom structure of a complex containing 10 molecules, of the N protein and 90 bases of RNA. The RNA is tightly sequestered in a, cavity at the interface between two lobes of the N protein. This serves to, protect the RNA in the absence of polynucleotide synthesis. For the RNA to, be accessed, some conformational change in the N protein should be, necessary.
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<StructureSection load='2gic' size='340' side='right'caption='[[2gic]], [[Resolution|resolution]] 2.92&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2gic]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.92&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gic OCA], [https://pdbe.org/2gic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gic RCSB], [https://www.ebi.ac.uk/pdbsum/2gic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gic ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NCAP_VSIVA NCAP_VSIVA] Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gi/2gic_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gic ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2GIC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus] with IUM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GIC OCA].
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*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of the vesicular stomatitis virus nucleoprotein-RNA complex., Green TJ, Zhang X, Wertz GW, Luo M, Science. 2006 Jul 21;313(5785):357-60. Epub 2006 Jun 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16778022 16778022]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Vesicular stomatitis Indiana virus]]
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[[Category: Vesicular stomatitis indiana virus]]
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[[Category: Green TJ]]
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[[Category: Green, T.J.]]
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[[Category: Luo M]]
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[[Category: Luo, M.]]
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[[Category: Wertz GW]]
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[[Category: Wertz, G.W.]]
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[[Category: Zhang X]]
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[[Category: Zhang, X.]]
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[[Category: IUM]]
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[[Category: negative strand virus]]
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[[Category: nucleocapsid]]
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[[Category: protein-rna complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:13:04 2007''
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Current revision

Crystal Structure of a vesicular stomatitis virus nucleocapsid-RNA complex

PDB ID 2gic

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