2gim

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2gim" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gim, resolution 1.60&Aring;" /> '''1.6 Angstrom structu...)
Current revision (09:41, 30 August 2023) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2gim.gif|left|200px]]<br /><applet load="2gim" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2gim, resolution 1.60&Aring;" />
 
-
'''1.6 Angstrom structure of plastocyanin from Anabaena variabilis'''<br />
 
-
==Overview==
+
==1.6 Angstrom structure of plastocyanin from Anabaena variabilis==
-
Plastocyanin from the cyanobacterium Anabaena variabilis was, heterologously produced in Escherichia coli and purified. Plate-like, crystals were obtained by crystallization in 1.15 M trisodium citrate and, 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized, protein was solved to a resolution of 1.6 Angstrom using plastocyanin from, Phormidium laminosum as a search model. Two molecules were found in the, asymmetric unit. The electrostatic surface of the basic protein showed a, large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged, patches. The copper ion was found to be relatively mobile and there were, two distinct conformations of His61.
+
<StructureSection load='2gim' size='340' side='right'caption='[[2gim]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2gim]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trichormus_variabilis Trichormus variabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GIM FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gim OCA], [https://pdbe.org/2gim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gim RCSB], [https://www.ebi.ac.uk/pdbsum/2gim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gim ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PLAS_ANAVA PLAS_ANAVA] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gi/2gim_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gim ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Plastocyanin from the cyanobacterium Anabaena variabilis was heterologously produced in Escherichia coli and purified. Plate-like crystals were obtained by crystallization in 1.15 M trisodium citrate and 7.67 mM sodium borate buffer pH 8.5. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.85, b = 45.81, c = 63.41 Angstrom. The structure of the oxidized protein was solved to a resolution of 1.6 Angstrom using plastocyanin from Phormidium laminosum as a search model. Two molecules were found in the asymmetric unit. The electrostatic surface of the basic protein showed a large population of positively charged residues in the northern site, whereas the eastern site lacked the two strongly negatively charged patches. The copper ion was found to be relatively mobile and there were two distinct conformations of His61.
-
==About this Structure==
+
Structure of plastocyanin from the cyanobacterium Anabaena variabilis.,Schmidt L, Christensen HE, Harris P Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:16929103<ref>PMID:16929103</ref>
-
2GIM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_variabilis Anabaena variabilis] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GIM OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure of plastocyanin from the cyanobacterium Anabaena variabilis., Schmidt L, Christensen HE, Harris P, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1022-9. Epub 2006, Aug 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16929103 16929103]
+
</div>
-
[[Category: Anabaena variabilis]]
+
<div class="pdbe-citations 2gim" style="background-color:#fffaf0;"></div>
-
[[Category: Single protein]]
+
-
[[Category: Christensen, H.E.M.]]
+
-
[[Category: Harris, P.]]
+
-
[[Category: Schmidt, L.]]
+
-
[[Category: CU]]
+
-
[[Category: beta sheet]]
+
-
[[Category: cu]]
+
-
[[Category: helix]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 11:13:29 2007''
+
==See Also==
 +
*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Trichormus variabilis]]
 +
[[Category: Christensen HEM]]
 +
[[Category: Harris P]]
 +
[[Category: Schmidt L]]

Current revision

1.6 Angstrom structure of plastocyanin from Anabaena variabilis

PDB ID 2gim

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gim"
Personal tools